<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Introduction: Multidrug-resistant tuberculosis (MDR-TB) represents an important public health problem. Rapid molecular methods detect the mutations responsible for drug- resistance and can shorten the time required for diagnosis and initiation of appropriate therapy. The objectives of our study were to optimize a multiplex allele-specific PCR technique (MAS-PCR) to detect rpoB mutations and to specify the type and frequency of these mutations. Material and methods: We have tested 47 non-duplicate MDR-TB, including extensively drugresistant tuberculosis (XDR-TB) strains, identified during 2007-2012, using MAS-PCR for detection of mutations in rpoB codons 531, 526 and 516. Results: The most common mutation was located in codon 531 (63.82%), while only 12.76% and 10.63% of the strains showed mutations in codon 516 and 526, respectively; for six strains we have not identified mutation in the targeted codons. Conclusion: MAS-PCR revealed the mutations prevalent in our region, with good sensitivity (87.2%), suggesting the usefulness of this test in the rapid diagnosis of MDR-TB</p></abstract>

Introduction: Multidrug-resistant tuberculosis (MDR-TB) represents an important public health problem. Rapid molecular methods detect the mutations responsible for drug- resistance and can shorten the time required for diagnosis and initiation of appropriate therapy. The objectives of our study were to optimize a multiplex allele-specific PCR technique (MAS-PCR) to detect rpoB mutations and to specify the type and frequency of these mutations. Material and methods: We have tested 47 non-duplicate MDR-TB, including extensively drugresistant tuberculosis (XDR-TB) strains, identified during 2007-2012, using MAS-PCR for detection of mutations in rpoB codons 531, 526 and 516. Results: The most common mutation was located in codon 531 (63.82%), while only 12.76% and 10.63% of the strains showed mutations in codon 516 and 526, respectively; for six strains we have not identified mutation in the targeted codons. Conclusion: MAS-PCR revealed the mutations prevalent in our region, with good sensitivity (87.2%), suggesting the usefulness of this test in the rapid diagnosis of MDR-TB

Molecular detection of rifampicin resistance in multidrug-resistant Mycobacterium tuberculosis strains from North-Eastern Romania

“Grigore T. Popa” University of Medicine and Pharmacy Iaşi, Microbiology Department

“St. Parascheva” Clinical Hospital of Infectious Diseases Iaşi

“St. Spiridon” Clinical Emergency Hospital Iaşi

National Institute of Public Health – Regional Center of Public Health Iaşi

Revista Romana de Medicina de Laborator

Introduction: Multidrug-resistant tuberculosis (MDR-TB) represents an important public health problem. Rapid molecular methods detect the mutations responsible...

Molecular detection of rifampicin resistance in multidrug-resistant Mycobacterium tuberculosis strains from North-Eastern Romania

Pubblicato online: 19 set 2013

Pagine: 293 - 299

DOI: https://doi.org/10.2478/rrlm-2013-0027

This content is open access.