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Correlation (R) between permeabilization and release of damage-associated molecular pattern molecules (DAMPs) after pulse treatment. Investigated time points for each molecule are present in the bottom row. Correlation was evaluated with Pearson correlation coefficient and permeabilization was analyzed by propidium iodide (PI) assay 3 minutes after pulse treatment
R vs permeabilization (PI)
MTS
-0.680
ATP
0.704
0.728 (L)/0.594 (F)
-0.695 (F)
DNA
0.571
0.689
NS
RNA
0.584
0.696
NS
CRT
0.535
0.556
uric acid
NS
time after points EP
3 min
15 min
30 min
4 h
24 h
Correlation (R) between survival and release of damage-associated molecular pattern molecules (DAMPs) after pulse treatment. Investigated time points for each molecule are presented in the bottom row. Correlation was evaluated with Pearson correlation coefficient and survival was analyzed via MTS assay 24 hours after pulse treatment