[
1. Sandberg Y, van Gastel-Mol EJ, Verhaaf B, Lam KH, van Dongen JJ, Langerak AW. BIOMED-2 multiplex immunoglobulin/T-cell receptor polymerase chain reaction protocols can reliably replace Southern blot analysis in routine clonality diagnostics. J Mol Diagn 2005; 7: 495-503.10.1016/S1525-1578(10)60580-6
]Search in Google Scholar
[
2. Hoeve MA, Krol AD, Philippo K, Derksen PW, Veenendaal RA, Schuuring E, et al. Limitations of clonality analysis of B cell proliferations using CDR3 polymerase chain reaction. J Clin Pathol Mol Pathol 2000; 53: 194-200.10.1136/mp.53.4.194
]Search in Google Scholar
[
3. van Krieken JH, Langerak AW, Macintyre EA, Kneba M, Hodges E, Garcia Sanz R, et al. Improved reliability of lymphoma diagnostics via PCR-based clonality testing: - Report of the BIOMED-2 concerted action BHM4-CT98-3936. Leukemia 2007; 21: 201-6.10.1038/sj.leu.2404467
]Search in Google Scholar
[
4. McCarthy KP, Sloane JP, Wiedemann LM. Rapid method for distinguishing clonal from polyclonal B cell populations in surgical biopsy specimens. J Clin Pathol 1990; 43: 429-32.10.1136/jcp.43.5.429
]Search in Google Scholar
[
5. Trainor KJ, Brisco MJ, Wan JH, Neoh S, Grist S, Morley AA. Gene rearrangement in B- and T-lymphoproliferative disease detected by the polymerase chain reaction. Blood 1991; 78: 192-5.10.1182/blood.V78.1.192.192
]Search in Google Scholar
[
6. Slack DN, McCarthy KP, Wiedemann LM, Sloane JP. Evaluation of sensitivity, specificity and reproducibility of an optimized method for detecting clonal rearrangements of immunoglobulin and T-cell receptor genes in formalinfixed, paraffin-embedded sections. Diagn Mol Pathol 1993; 2: 223-32.10.1097/00019606-199312000-00001
]Search in Google Scholar
[
7. Segal GH, Jorgensen T, Masih AS, Braylan TC. Optimal primer selection for clonality assessment by polymerase chain reaction analysis: I. Low grade B-cell lymphoproliferative disorders of nonfollicular center cell type. Hum Pathol 1994; 25: 1269-75.10.1016/0046-8177(94)90084-1
]Search in Google Scholar
[
8. Davis TH, Courtland EY, Balk SP. Detection of clonal immunoglobulin gene rearrangements by polymerase chain reaction amplification and single-strand conformational polymorphism analysis. Am J Pathol 1993; 142: 1841-47.
]Search in Google Scholar
[
9. Chen YT, Whitney KD, Chen Y. Clonality analysis of B-cell lymphoma in freshfrozen and paraffin-embedded tissues: The effects of variable polymerase chain reaction parameters. Mod Pathol 1994; 7: 429-34.
]Search in Google Scholar
[
10. McCarthy KP, Sloane JP, Wiedemann LM. A simplified method of detection of clonal rearrangements of the T-cell receptor g chain gene. Diagn Mol Pathol 1992; 1: 173-79.
]Search in Google Scholar
[
11. Theodorou I, Raphael M, Bigorgne C, Fourcade C, Lahet C, Cochet G, et al. Recombination pattern of the TCRg locus in human peripheral T-cell lymphomas. J Pathol 1994; 174: 233-42.10.1002/path.17117404027884584
]Search in Google Scholar
[
12. Diss TC, Watts M, Pan LX, Burke M, Linch D, Isaacson PG. The polymerase chain reaction in the demonstration of monoclonality in T cell lymphomas. J Clin Pathol 1995; 48: 1045-50.10.1136/jcp.48.11.10455030128543629
]Search in Google Scholar
[
13. Liu H, Bench AJ, Bacon CM, Payne K, Huang Y, Scott MA, et al. A practical strategy for the routine use of BIOMED-2 PCR assays for detection of B- and T-cell clonality in diagnostic haematopathology. Br J Haematol 2007; 138: 31-43.10.1111/j.1365-2141.2007.06618.x17555445
]Search in Google Scholar
[
14. van Dongen JJ, Langerak AW, Brüggemann M, Evans PA, Hummel M, Lavender FL, et al. Design and standardization of PCR primers and protocols for detection of clonal immunoglobulin and T-cell receptore gene recombinations in suspect lymphoproliferations: report of the BIOMED-2 Concerted Action BHM4-CT98-3936. Leukemia 2003; 17: 2257-317.10.1038/sj.leu.2403202
]Search in Google Scholar
[
15. Evans PA, Pott C, Groenen PJ, Salles G, Davi F, Berger F, et al. Significantly improved PCR-based clonality testing in B-cell malignancies by use of multiple immunoglobulin gene targets. Report of the BIOMED-2 Concerted Action BHM4-CT98-3936. Leukemia 2007; 21: 207-14.10.1038/sj.leu.2404479
]Search in Google Scholar
[
16. Brüggemann M, White H, Gaulard P, Garcia-Sanz R, Gameiro P, Oeschger S, et al. Powerful strategy for polymerase chain reaction-based clonality assessment in T-cell malignancies. Report of the BIOMED-2 Concerted Action BHM4-CT98-3936. Leukemia 2007; 21: 215-21.10.1038/sj.leu.2404481
]Search in Google Scholar
[
17. Langerak AW, Molina TJ, Lavender FL, Pearson D, Flohr T, Sambade C, et al. Polymerase chain reaction-based clonality testing in tissue samples with reactive lymphoproliferations: usefulness and pitfalls. A report of the BIOMED-2 Concerted Action BHM4-CT98-3936. Leukemia 2007; 21: 222-29.10.1038/sj.leu.2404482
]Search in Google Scholar
[
18. Langerak AW, Groenen PJ, van Krieken JH, van Dongen JJ. Immunoglobulin/Tcell receptor clonality diagnostics. Expert Opin Med Diagn 2007; 1: 451-61.10.1517/17530059.1.4.451
]Search in Google Scholar
[
19. Groenen PJ, Langerak AW, van Dongen JJ, van Krieken JH. Pitfalls in TCR gene clonality testing: teaching cases. J Hematopathol 2008; 1: 97-109.10.1007/s12308-008-0013-9
]Search in Google Scholar
[
20. Matthews C, Catherwood M, Morris TC, Alexander HD. Routine analysis of IgHV mutational status in CLL patients using BIOMED-2 standardized primers and protocols. Leuk Lymphoma 2004; 45: 1899-904.10.1080/10428190410001710812
]Search in Google Scholar
[
21. Dawidovska M, Jólkowska J, Sczepański T, Derwich K, Wachowiak J, Witt M. Implementation of the standard strategy for identification of Ig/TCR targets for minimal residual disease diagnostics in B-cell precursor ALL pediatric patients: Polish experience. Arch Immunol Ther Exp 2008; 56: 409-18.10.1007/s00005-008-0045-y
]Search in Google Scholar
[
22. Chen YL, Su IJ, Cheng HY, Chang KC, Lu CC, Chow NH, et al. BIOMED-2 protocols to detect clonal immunoglobulin and T-cell receptor gene rearrangements in B- and T-cell lymphomas in southern Taiwan. Leuk Lymphoma 2010; 51: 650-55.10.3109/10428191003660631
]Search in Google Scholar
[
23. Lassmann S, Gerlach UV, Technau-Ihling K, Werner M, Fisch P. Application of BIOMED-2 primers in fixed and decalcified bone marrow biopsies: analysis of immunoglobulin H receptor rearrangements in B-cell non-Hodgkin’s lymphomas. J Mol Diagn 2005; 7: 582-91.10.1016/S1525-1578(10)60591-0
]Search in Google Scholar
[
24. Lukowsky A, Marchwat M, Sterry W, Gellrich S. Evaluation of B-cell clonality in archival skin biopsy samples of cutaneous B-cell lymphoma by immunoglobulin heavy chain gene polymerase chain reaction. Leuk Lymphoma 2006; 47: 487-93.10.1080/1042819050030538016396773
]Search in Google Scholar
[
25. Hebeda KM, van Altena MC, Rombout P, van Krieken JH, Groenen PJ. PCR clonality detection in Hodgkin lymphoma. J Hematopathol 2009;2:34-41. 10.1007/s12308-009-0024-1271349219669221
]Search in Google Scholar
[
26. Burack WR, Laughlin TS, Friedberg JW, Spence JM, Rothberg PG. PCR assays detect B-lymphocyte clonality in formalin-fixed, paraffin-embedded specimens of classical Hodgkin lymphoma without microdissection. Am J Clin Pathol 2010; 134: 104-11.10.1309/AJCPK6SBE0XOODHB304678020551274
]Search in Google Scholar
[
27. Zhang S, Abreo F, Lowery-Nordberg M, Veillon DM, Cotelingam JD. The role of fluorescence in situ hybridization and polymerase chain reaction in the diagnosis and classification of lymphoproliferative disorders on fine-needle aspiration. Cancer Cytopathol 2010; 118: 105-12.10.1002/cncy.2007020340097
]Search in Google Scholar
[
28. Payne K, Wright P, Grant JW, Huang Y, Hamoudi J, Bacon CM, et al. BIOMED-2 PCR assays for IGK gene rearrangements are essential for B-cell clonality analysis in follicular lymphoma. Br J Haematol 2011; 155: 84-92.10.1111/j.1365-2141.2011.08803.x21790530
]Search in Google Scholar
[
29. Berget E, Helgeland L, Molven A, Vintermyr OK. Detection of clonality in follicular lymphoma using formalin-fixed, paraffin-embedded tissue samples and BIOMED-2 immunoglobulin primers. J Clin Pathol 2011; 64: 37-41.10.1136/jcp.2010.08110921030528
]Search in Google Scholar
[
30. Sandberg Y, Heule F, Lam K, Lugtenburg PJ, Wolvers-Tettero IL, van Dongen JJ, et al. Molecular immunoglobulin/T-cell receptor clonality in cutaneous lymphoproliferations. Experience with the BIOMED-2 standardized polymerase chain reaction protocol. Haematologica 2003; 88: 659-70.
]Search in Google Scholar
[
31. Catherwood MA, Gonzales D, Patton C; Dobbin E, Venkatraman L, Alexander HD. Improved clonality assessment in germinal centre/post germinal centre non- Hodgkin’s lymphomas with high rates of somatic hypermutation. J Clin Pathol 2007; 60: 524-28.
]Search in Google Scholar
[
32. Tan BT, Seo K, Warnke RA, Arber DA. The frequency of immunoglobulin heavy chain gene and T-cell receptor g-chain gene rearrangements and Epstein-Barr virus in ALK+ and ALK- anaplastic large cell lymphoma and other peripheral T-cell lymphomas. J Mol Diagn 2008; 10: 502-12.10.2353/jmoldx.2008.080054257063318832464
]Search in Google Scholar
[
33. Zhang B, Beck AH, Taube JM, Kohler S, Seo K, Zwerner J, et al. Combined use of PCR-based TCRG and TCRB clonality tests on paraffin-embedded skin tissue in the differential diagnosis of Mycosis fungoides and inflammatory dermatoses. J Mol Diagn 2010; 12: 320-27.10.2353/jmoldx.2010.090123286046820203005
]Search in Google Scholar
[
34. Nakamichi N, Wada N, Kohara M, Fukuhara S, Sugiyama H, Ogawa H, et al. Polymorphous lymphoproliferative disorder: a clinicopathological analysis.Virchows Arch 2010; 456: 269-76.10.1007/s00428-010-0880-120111873
]Search in Google Scholar
[
35. Dabiri S, Morales A, Ma L, Sundram U, Kim YH, Arber DA, et al. The frequency of dual TCR-PCR clonality in granulomatous disorders. J Cutan Pathol 2011; 38: 704-9.10.1111/j.1600-0560.2011.01727.x21645036
]Search in Google Scholar
[
36. McClure RF, Kaur P, Pagel E, Ouillette PD, Holtegaard CE, Treptow CL, et al. Validation of immunoglobulin gene rearrangement detection by PCR using commercially available BIOMED-2 primers. Leukemia 2006; 20: 176-79.10.1038/sj.leu.240404916307010
]Search in Google Scholar
[
37. Patel KP, Pan Q, Wang Y, Maitta RW; Du J, Xue X, et al. Comparison of BIOMED-2 versus laboratory-developed polymerase chain reaction assays for detecting T-cell receptor-g gene rearrangements. J Mol Diagn 2010; 12: 226-37.10.2353/jmoldx.2010.090042287173020181819
]Search in Google Scholar
[
38. Zaki MA, Wada N, Kohara M, Ikeda J, Hori Y, Fujita S, et al. Presence of B-cell clones in T-cell lymphoma. Eur J Haematol 2011; 86: 412-19.10.1111/j.1600-0609.2011.01597.x21362051
]Search in Google Scholar
[
39. Langerak AW, Groenen PJ, Brüggemann M, Beldjord K, Bellan C, Bonello L, et al. EuroClonality/BIOMED-2 guidelines for interpretation and reporting of Ig/TCR clonality testing in suspected lymphoproliferations. Leukemia 2012; 26: 2159-71. 10.1038/leu.2012.246346978922918122
]Search in Google Scholar
[
40. Jaffe ES, Harris NL, Stein H and Vardiman JW: Tumours of Haematopoietic and Lymphoid Tissues. In: World Health Organization Classification of Tumours. Lyon: IARC Press; 2001.
]Search in Google Scholar
[
41. Swerdlow SH, Campo E, Harris NL, Jaffe ES, Pileri SA, Stein H, et al. WHO Classification of Tumors of Haematopoietic and Lymphoid Tissues. Lyon: IARC Press; 2008.
]Search in Google Scholar
[
42. Diss TC, Peng H, Wotherspoon AC, Isaacson PG, Pan L. Detection of monoclonality in low-grade B-cell lymphomas using the polymerase chain reaction is dependent on primer selection and lymphoma type. J Pathol 1993; 169: 291-95.10.1002/path.17116903038492220
]Search in Google Scholar
[
43. Posnett DN, Sinha R, Kabak S, Russo C. Clonal populations of T cells in normal elderly humans: the T cell equivalent to “benign monoclonal gammapathy”. J Exp Med 1994; 179: 609-18.10.1084/jem.179.2.60921913748294871
]Search in Google Scholar
[
44. Delfau-Larue MH, Laroche L, Wechsler J, Lepage E, Lahet C, Asso-Bonnet M, et al. Diagnostic value of dominant T-cell clones in peripheral blood in 363 patients presenting consecutively with a clinical suspicion of cutaneous lymphoma. Blood 2000; 96: 2987-92.10.1182/blood.V96.9.2987
]Search in Google Scholar
[
45. Godges E, Krishna MT, Pickard C, Smith JL. Diagnostic role of test for T cell receptor (TCR) genes. J Clin Pathol 2003; 56: 1-11. 10.1136/jcp.56.1.1176986512499424
]Search in Google Scholar
