We recorded vocal interaction in the natural environment of an Eurasian Eagle-Owl (Bubo bubo) with canines Gray Wolves (Canis lupus), Red Foxes (Vulpes vulpes) and domestic dogs (Canis familiaris). Vocalization was recorded using Olympus digital voice recorders. The calls of the male Eurasian Eagle-Owl were recorded by us in the frequency range of 200–420 Hz. The howl of a Gray Wolf was recorded in the frequency range from 300 to 1,100 Hz. Red Fox barking was recorded in the frequency range from 750 to 1,000 Hz. Barking of domestic dogs was recorded in the frequency range from 250 to 1,500 Hz. The vocalization of the Eurasian Eagle-Owl had an independent character inherent in the biology of the species. The Eurasian Eagle-Owl, with its cries, involuntarily provoked the entry of canines into joint vocal interaction, which can be explained by the high social activity of the latter. Co-vocalizations of the Eurasian Eagle-Owl and canines were noted in winter, spring and autumn, but mainly in spring (50%). The increased use of autonomous voice recorders, which record spontaneous vocalizations emitted by animals over long periods, will allow us to better document and study the importance of such interspecific interactions.
