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This study explores the activity of total superoxide dismutase (SOD), ascorbate peroxidase (APX) and glutathione reductase (GR), biomass accumulation and chlorophyll <italic>a</italic> content in <italic>Scenedesmus ellipsoideus</italic> Chodat grown under conditions of varying zinc (Zn) concentrations. In addition, the activity of different SOD isozymes (MnSOD, FeSOD and CuZnSOD) was measured separately to determine the intracellular extent of oxidative stress resulting from Zn toxicity. We found that the activity of FeSOD and MnSOD was induced by lower Zn concentration (2 μg ml−1 and 4 μg ml−1, respectively), whereas CuZnSOD activity was not affected, which indicates that chloroplasts are the first location in <italic>S. ellipsoideus</italic> cells where superoxide accumulation is accelerated by Zn toxicity. The activity of total SOD and APX was significantly increased by moderate Zn concentrations, probably due to some oxidative stress caused by Zn toxicity. The higher level of Zn application, however, led not only to the inhibition of total SOD and APX activity, but also to the reduction of biomass accumulation and chlorophyll <italic>a</italic> content. As a result, it can be concluded that the accumulation of superoxide radicals and H2O2 in <italic>S. ellipsoideus</italic> cells induced by Zn toxicity may be responsible for the reduced growth rate and the impairment of photosynthetic pigments.</abstract>

This study explores the activity of total superoxide dismutase (SOD), ascorbate peroxidase (APX) and glutathione reductase (GR), biomass accumulation and chlorophyll a content in Scenedesmus ellipsoideus Chodat grown under conditions of varying zinc (Zn) concentrations. In addition, the activity of different SOD isozymes (MnSOD, FeSOD and CuZnSOD) was measured separately to determine the intracellular extent of oxidative stress resulting from Zn toxicity. We found that the activity of FeSOD and MnSOD was induced by lower Zn concentration (2 μg ml−1 and 4 μg ml−1, respectively), whereas CuZnSOD activity was not affected, which indicates that chloroplasts are the first location in S. ellipsoideus cells where superoxide accumulation is accelerated by Zn toxicity. The activity of total SOD and APX was significantly increased by moderate Zn concentrations, probably due to some oxidative stress caused by Zn toxicity. The higher level of Zn application, however, led not only to the inhibition of total SOD and APX activity, but also to the reduction of biomass accumulation and chlorophyll a content. As a result, it can be concluded that the accumulation of superoxide radicals and H2O2 in S. ellipsoideus cells induced by Zn toxicity may be responsible for the reduced growth rate and the impairment of photosynthetic pigments.

Assessment of the effects of zinc on the growth and antioxidant enzymes in <italic>Scenedesmus ellipsoideus</italic> Chodat

Oceanological and Hydrobiological Studies

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Assessment of the effects of zinc on the growth and antioxidant enzymes in  Chodat

This study explores the activity of total superoxide dismutase (SOD), ascorbate peroxidase (APX) and glutathione reductase (GR), biomass accumulation and...

Assessment of the effects of zinc on the growth and antioxidant enzymes in Scenedesmus ellipsoideus Chodat

Article Category: Original research paper

Pubblicato online: 22 set 2019

Pagine: 270 - 278

Ricevuto: 17 gen 2019

Accettato: 11 mar 2019

DOI: https://doi.org/10.2478/ohs-2019-0024

© 2019 Faculty of Oceanography and Geography, University of Gdańsk, Poland

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.