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The use of gamma irradiation to stimulate bioactive compound synthesis in Inonotus obliquus submerged cultures

Alexandru Petre
Alexandru Petre
, 
Mihaela Ene
Mihaela Ene
, 
Daniel Constantin Negut
Daniel Constantin Negut
, 
Florentina Gatea
Florentina Gatea
 e 
Emanuel Vamanu
Emanuel Vamanu
   | 17 set 2021
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Article Category: Original Paper
Pubblicato online: 17 set 2021
Pagine: 83 - 90
Ricevuto: 02 feb 2021
Accettato: 07 giu 2021
DOI: https://doi.org/10.2478/nuka-2021-0012
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© 2021 Alexandru Petre et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1

The radiation dose-dependent distribution of dry mycelium weight per 400 mL of culture, in the first experimental run.
The radiation dose-dependent distribution of dry mycelium weight per 400 mL of culture, in the first experimental run.

Fig. 2

The radiation dose-dependent distribution of dry mycelium weight per 400 mL of culture, in the second experimental run.
The radiation dose-dependent distribution of dry mycelium weight per 400 mL of culture, in the second experimental run.

Fig. 3

The radiation dose-dependent distribution of free radical scavenging potential of the mycelium extracts.
The radiation dose-dependent distribution of free radical scavenging potential of the mycelium extracts.

Fig. 4

The radiation dose-dependent distribution of total phenolic content of the mycelium extracts.
The radiation dose-dependent distribution of total phenolic content of the mycelium extracts.

Fig. 5

The radiation dose-dependent distribution of total phenolic content of the fermented broths.
The radiation dose-dependent distribution of total phenolic content of the fermented broths.

Fig. 6

The radiation dose-dependent distribution of total flavonoid content of the fermented broths.
The radiation dose-dependent distribution of total flavonoid content of the fermented broths.

Fig. 7

The radiation dose-dependent distribution of sinapinic acid content of the fermentation broths.
The radiation dose-dependent distribution of sinapinic acid content of the fermentation broths.

The one-way ANOVA of total phenolic content of the mycelium extracts

Groups Count Sum Average Variance Std. deviation Std. error
0 Gy 5 439.4212 87.8842 98.0936 9.9042 4.4293
100 Gy 4 451.3970 112.8492 124.0126 11.1361 5.5680
200 Gy 5 632.8615 126.5723 76.2878 8.7343 3.9061
300 Gy 5 684.2167 136.8433 417.0659 20.4222 9.1331
400 Gy 5 667.4257 133.4851 238.70581 15.4501 6.9095
ANOVA single factor (α = 0.01)
Source of variation SS df MS F P-value F crit
Between groups 7 904.44478 4 1 976.111195 10.16779312 0.00014191 4.500257699
Within groups 3 692.651123 19 194.3500591
Total 11 597.0959 23

The one-way ANOVA of total flavonoid content of the fermented broths

Groups Count Sum Average Variance Std. deviation Std. error
0 Gy 5 0.5825 0.1165 0.00007 0.008539 0.003819
100 Gy 5 0.9134 0.1826 0.00974 0.098740 0.044158
200 Gy 5 5.7376 1.1475 0.26596 0.515721 0.230637
300 Gy 5 5.9381 1.1876 0.10480 0.323743 0.144782
400 Gy 5 6.0272 1.2054 0.18652 0.431886 0.193145
ANOVA single factor (α = 0.01)
Source of variation SS df MS F P-value F crit.
Between groups 6.392584426 4 1.598146107 14.08987153 0.000012734 4.430690161
Within groups 2.268503447 20 0.113425172
Total 8.661087873 24

The one-way ANOVA of free radical scavenging potential of the mycelium extracts

Groups Count Sum Average Variance Std. deviation Std. error
0 Gy 5 1.3562 0.2712 0.0019 0.044382 0.019848
100 Gy 4 1.6489 0.4122 0.0011 0.034140 0.017070
200 Gy 5 2.1722 0.4344 0.0008 0.029030 0.012983
300 Gy 5 2.4386 0.4877 0.0025 0.050210 0.022455
400 Gy 5 2.3953 0.4790 0.0014 0.038588 0.017257
ANOVA single factor (α = 0.01)
Source of variation SS df MS F P-value F crit.
Between groups 0.152096309 4 0.038024077 23.466281 0.0000003965 4.500257699
Within groups 0.030787046 19 0.001620371
Total 0.182883355 23

The one-way ANOVA of total phenolic content of the fermented broths

Groups Count Sum Average Variance Std. deviation Std. error
0 Gy 5 73.6308 14.72610 4.2755 2.0678 0.9247
100 Gy 5 72.1087 14.42170 0.4407 0.6639 0.2969
200 Gy 5 103.1155 20.62311 7.9304 2.8161 1.2594
300 Gy 5 111.8099 22.36199 3.9634 1.9908 0.8903
400 Gy 5 120.0085 24.00170 15.6380 3.9545 1.7685
ANOVA single factor (α = 0.01)
Source of variation SS df MS F P-value F crit.
Between groups 389.6157862 4 97.403946550 15.10220151 0.000007715 4.430690161
Within groups 128.9930431 20 6.449652157
Total 518.6088293 24

The one-way ANOVA of dry mycelium weight after irradiation and culture of the first experimental run

Groups Count Sum Average Variance Std. deviation Std. error
0 Gy 5 6.78 1.356 0.00468 0.068411 0.030594
100 Gy 5 7.09 1.418 0.00342 0.058481 0.026153
200 Gy 5 7.73 1.546 0.00753 0.086776 0.038807
300 Gy 5 8.07 1.614 0.00863 0.092898 0.041545
400 Gy 5 8.12 1.624 0.00433 0.065803 0.029428
ANOVA single factor (α = 0.01)
Source of variation SS df MS F P-value F crit.
Between groups 0.286376 4 0.071594 12.52081147 0.000029182 4.430690161
Within groups 0.114360 20 0.005718
Total 0.400736 24

The one-way ANOVA of the sinapinic acid content of the fermentation broths

Groups Count Sum Average Variance Std. deviation Std. error
0 Gy 5 40.0005 8.0001 25.7390 5.073365 2.536682
100 Gy 3 51.3022 17.1007 26.4171 5.139765 3.634363
200 Gy 2 58.7856 29.3928 304.6469 20.619987 14.580533
300 Gy 3 91.1330 30.3776 18.1002 4.254438 3.008342
400 Gy 1 55.2323 55.2323 0 0 0
ANOVA single factor (α= 0.01)
Source of variation SS df MS F P-value F crit.
Between groups 2466.721606 4 616.6804015 11.17539311 0.001532718 6.422085458
Within groups 496.6378863 9 55.18198737
Total 2963.359492 13

The one-way ANOVA of dry mycelium weight after irradiation and culture of the second experimental run

Groups Count Sum Average Variance Std. deviation Std. error
0 Gy 5 9.20 1.840 0.00535 0.073144 0.032710
100 Gy 5 9.99 1.998 0.00452 0.067231 0.030066
200 Gy 5 10.49 2.098 0.00352 0.059330 0.026532
300 Gy 5 10.73 2.146 0.00843 0.091815 0.041060
400 Gy 5 10.35 2.070 0.00490 0.07 0.031304
ANOVA single factor (α= 0.01)
Source of variation SS df MS F P-value F crit.
Between groups 0.284016 4 0.071004 13.28667665 0.000019303 2.866081402
Within groups 0.106880 20 0.005344 0.10688
Total 0.390896 24
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