<abstract xmlns="http://www.w3.org/1999/xhtml"><p> The enzymatic deamination of 5-fl uorotryptamine and 5-hydroxytryptamine, 5-HT, catalysed by enzyme monoamine oxidase A (MAO-A, EC 1.4.3.4) was investigated using the kinetic (KIE) and solvent (SIE) isotope effects methods. The numerical values of deuterium isotope effects in the (1R) positions of 5-F-tryptamine were determined using non-competitive spectrophotomeric method. Isotopologue 5-F-[(1R)- -<sup>2</sup>H]-tryptamine, needed for kinetic studies was obtained by enzymatic decarboxylation of 5´-fl uoro-L-tryptophan, 5´-F-L-Trp, in fully deuteriated medium. </p></abstract>

The enzymatic deamination of 5-fl uorotryptamine and 5-hydroxytryptamine, 5-HT, catalysed by enzyme monoamine oxidase A (MAO-A, EC 1.4.3.4) was investigated using the kinetic (KIE) and solvent (SIE) isotope effects methods. The numerical values of deuterium isotope effects in the (1R) positions of 5-F-tryptamine were determined using non-competitive spectrophotomeric method. Isotopologue 5-F-[(1R)- -2H]-tryptamine, needed for kinetic studies was obtained by enzymatic decarboxylation of 5´-fl uoro-L-tryptophan, 5´-F-L-Trp, in fully deuteriated medium.

Enzymatic oxidation of substituted tryptamines catalysed by monoamine oxidase

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{"article-title":"Enzymatic oxidation of substituted tryptamines catalysed by monoamine oxidase"}

The enzymatic deamination of 5-fl uorotryptamine and 5-hydroxytryptamine, 5-HT, catalysed by enzyme monoamine oxidase A (MAO-A, EC 1.4.3.4) was investigated...

Enzymatic oxidation of substituted tryptamines catalysed by monoamine oxidase

Pubblicato online: 12 set 2014

Pagine: 91 - 95

Ricevuto: 01 mar 2013

Accettato: 26 giu 2014

DOI: https://doi.org/10.2478/nuka-2014-0015

Parole chiave
monoamine oxidase (MAO), tryptamine, serotonin, isotope effects

© by Marianna Kańska

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Enzymatic oxidation of substituted tryptamines catalysed by monoamine oxidase

Pubblicato online: 12 set 2014

Pagine: 91 - 95

Ricevuto: 01 mar 2013

Accettato: 26 giu 2014

DOI: https://doi.org/10.2478/nuka-2014-0015

Parole chiavemonoamine oxidase (MAO), tryptamine, serotonin, isotope effects

© by Marianna Kańska

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Parole chiave
monoamine oxidase (MAO), tryptamine, serotonin, isotope effects