Whole genome sequencing and analysis of a goose-derived Mycoplasma gallisepticum in Guangdong Province, China
Yuanyuan Zhou
Profilo Orcid, Shuti Song
Profilo Orcid, Weihuo Li
Profilo Orcid, Yixin Jia
Profilo Orcid, Yangshuo Li
Profilo Orcid, Jingyi Liang
Profilo Orcid, Zhaofeng Yao
Profilo Orcid e 
Pubblicato online: 28 nov 2024
Pagine: 497 - 508
Ricevuto: 18 apr 2024
Accettato: 21 nov 2024
DOI: https://doi.org/10.2478/jvetres-2024-0065
Parole chiave
© 2024 Yuanyuan Zhou et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Introduction
Material and Methods
A strain designated MG-GD01/22 was isolated from the air-sac tissues of five geese with chronic respiratory disease on a Guangdong goose farm. Its pathogenicity was assessed, antimicrobial susceptibility tests were performed using agar dilution, and its total DNA was extracted for whole-genome sequencing and gene function annotation with second- and third-generation sequencing technologies. The homology of the 16S ribosomal RNA (rRNA) region was analysed and a phylogenetic tree was constructed, as was an evolutionary tree of the
Results
The isolate produced “fried egg” colonies and was pathogenic to goslings. It was resistant to enrofloxacin, danofloxacin and spectinomycin and susceptible to valnemulin, tilmicosin, tylosin, acetylisovaleryltylosin tartrate and tiamulin. The genome analysis revealed 1,666 coding genes. Gene database annotation identified 25 virulence-related genes, 22 drug resistance–related genes, 13 pathogen-host–interaction genes and 9 carbohydrate-active enzyme genes. The isolate exhibited 99.9% homology to the MG S6 strain by its 16S rRNA, while the
Conclusion
This study offers theoretical references for the diagnosis, prevention and treatment of MG in geese in the Guangdong region.