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Distribution and neurochemical characterisation of neurons containing neuregulin 1 in the enteric nervous system within the porcine small intestine

Łukasz Puchała

Łukasz Puchała

4Pharma sp. z o.o.Warszawa, Poland
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Puchała, Łukasz
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Sławomir Gonkowski

Sławomir Gonkowski

Department of Clinical PhysiologyOlsztyn, Poland
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Gonkowski, Sławomir
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Liliana Rytel

Liliana Rytel

Department of Internal Diseases with Clinic, Faculty of Veterinary Medicine, University of Warmia and MazuryOlsztyn, Poland
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Joanna Wojtkiewicz

Joanna Wojtkiewicz

Department of Physiology and Pathophysiology, School of Medicine, University of Warmia and MazuryOlsztyn, Poland
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Wojtkiewicz, Joanna
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Waldemar Jarosław Grzegorzewski

Waldemar Jarosław Grzegorzewski

Institute of Biology, College of Natural Sciences, University of RzeszówRzeszów, Poland
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Grzegorzewski, Waldemar Jarosław
  
06 nov 2024
Distribution and neurochemical characterisation of neurons containing neuregulin 1 in the enteric nervous system within the porcine small intestine's Cover Image
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Pubblicato online: 06 nov 2024
Pagine: 623 - 632
Ricevuto: 04 giu 2024
Accettato: 29 ott 2024
DOI: https://doi.org/10.2478/jvetres-2024-0063
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© 2024 Łukasz Puchała et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1.

Example micrographs of neuregulin 1 (NRG 1)-positive neurons in the duodenal myenteric plexus (I), jejunal outer submucous plexus (II) and ileal inner submucous plexus (III). Arrows – neurons immunoreactive to both the anti–protein gene product (PGP) 9.5 and anti-NRG 1 antibodies; scale bar – 20 μm
Example micrographs of neuregulin 1 (NRG 1)-positive neurons in the duodenal myenteric plexus (I), jejunal outer submucous plexus (II) and ileal inner submucous plexus (III). Arrows – neurons immunoreactive to both the anti–protein gene product (PGP) 9.5 and anti-NRG 1 antibodies; scale bar – 20 μm

Fig. 2.

Example micrographs of the colocalisation of vasoactive intestinal polypeptide (VIP) and neuregulin 1 (NRG 1) in neurons located in the duodenal outer submucous plexus (I), jejunal inner submucous plexus (II) and ileal myenteric plexus (III). Arrows – neurons immunoreactive to both the anti-VIP and anti-NRG1 antibodies; scale bar – 20 μm
Example micrographs of the colocalisation of vasoactive intestinal polypeptide (VIP) and neuregulin 1 (NRG 1) in neurons located in the duodenal outer submucous plexus (I), jejunal inner submucous plexus (II) and ileal myenteric plexus (III). Arrows – neurons immunoreactive to both the anti-VIP and anti-NRG1 antibodies; scale bar – 20 μm

Fig. 3.

Example micrographs of the colocalisation of galanin (GAL) and neuregulin 1 (NRG 1) in neurons located in the duodenal myenteric plexus (I), jejunal inner submucous plexus (II) and ileal outer submucous plexus (III). Arrows – neurons immunoreactive to both the anti-GAL and anti-NRG1 antibodies; scale bar – 20 μm
Example micrographs of the colocalisation of galanin (GAL) and neuregulin 1 (NRG 1) in neurons located in the duodenal myenteric plexus (I), jejunal inner submucous plexus (II) and ileal outer submucous plexus (III). Arrows – neurons immunoreactive to both the anti-GAL and anti-NRG1 antibodies; scale bar – 20 μm

Fig. 4.

Example micrographs of the colocalisation of the neuronal isoform of nitric oxide synthase (nNOS) and neuregulin 1 (NRG 1) in neurons located in the duodenal outer submucous plexus (I), jejunal inner submucous plexus (II) and ileal myenteric plexus (III). Arrows – neurons immunoreactive to both the anti-nNOS and anti-NRG 1 antibodies; scale bar – 20 μm
Example micrographs of the colocalisation of the neuronal isoform of nitric oxide synthase (nNOS) and neuregulin 1 (NRG 1) in neurons located in the duodenal outer submucous plexus (I), jejunal inner submucous plexus (II) and ileal myenteric plexus (III). Arrows – neurons immunoreactive to both the anti-nNOS and anti-NRG 1 antibodies; scale bar – 20 μm

The list of antisera and reagents used in immunohistochemical investigations

Primary antibodies
Antigen Code Species Working dilution Supplier
GAL T-5036 Guinea pig 1 : 2,000 Peninsula Labs, San Carlos, CA, USA
nNOS N218 Mouse 1 : 1,000 Sigma-Aldrich, Saint Louis, MO, USA
NRG 1 AA 198–229 Rabbit 1 : 1,000 Antibodies-online, Aachen, Germany
PGP 9.5 7863-2004 Mouse 1 : 1,000 BioRad, Hercules, CA, USA
VIP 9535-0504 Mouse 1 : 1,000 BioRad
Secondary antibodies
Working dilution Supplier
Alexa Fluor 488 donkey anti-rabbit IgG 1 : 1,000 ThermoFisher Scientific Waltham, MA, USA
Alexa Fluor 546 donkey anti-mouse IgG 1 : 1,000 ThermoFisher Scientific
Alexa Fluor 546 donkey anti-guinea pig IgG 1 : 1,000 ThermoFisher Scientific

The degree of colocalisation of neuregulin 1 (NRG 1) and the neuronal isoform of nitric oxide synthase (nNOS) in the porcine small intestine

Enteric plexus Duodenum Jejunum Ileum
MP 17.03 ± 1.35 %a 31.04 ± 1.06 %b 31.77 ± 1.14 %b
OSP 26.25 ± 1.46 %a 31.43 ± 1.22 %b 29.52 ± 0.93 %b
ISP 18.82 ± 1.28 %a 15.96 ± 1.02 %a 16.96 ± 1.19 %a

The degree of colocalisation of neuregulin 1(NRG 1) and galanin (GAL) in the porcine small intestine

Enteric plexus Duodenum Jejunum Ileum
MP 15.67 ± 0.94%a 28.73 ± 0.81%b 26.38 ± 1.37%b
OSP 17.41 ± 1.00%a 30.53 ± 1.29%b 26.21 ± 0.84%c
ISP 11.47 ± 1.21%a 26.01 ± 1.10%b 15.17 ± 1.24%a

The percentage of neuregulin 1-positive neurons in the porcine small intestine

Enteric plexus Duodenum Jejunum Ileum
MP 21.52 ± 0.98%a 16.16 ± 0.88%b 18.48 ± 0.66%b
OSP 19.98 ± 0.69%a 16.26 ± 0.84%b 15.20 ± 0.63%b
ISP 16.92 ± 1.19%a 8.38 ± 0.55%b 10.26 ± 0.70%b

The degree of colocalisation of neuregulin 1 (NRG 1) and vasoactive intestinal polypeptide (VIP) in the porcine small intestine

Enteric plexus Duodenum Jejunum Ileum
MP 32.39 ± 1.82%a 25.50 ± 1.69%b 29.17 ± 1.45%a
OSP 41.91 ± 1.90%a 24.96 ±2.24%b 36.62 ± 1.43%c
ISP 25.16 ± 0.94%a 20.46 ± 1.23%b 23.55 ± 0.87%a
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Inglese
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Argomenti della rivista:
Scienze biologiche, Biologia molecolare, Microbiologia e virologia, Scienze della vita, altro, Medicina, Medicina veterinaria
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