<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec>
<h3>Introduction</h3>
<p>Bovine herpesvirus 6 (BoHV6) belongs to the <italic>Herpesviridae</italic> family, <italic>Gammaherpesvirinae</italic> subfamily and <italic>Macavirus</italic> genus. It is common in cattle, but was also detected in American bison <italic>(Bison bison)</italic> and water buffalo <italic>(Bubalus bubalis</italic>). The aim of the experiment was to develop an ELISA for serological examination of cattle sera for the presence of anti-BoHV6 specific antibodies.</p>
</sec>
<sec>
<h3>Material and Methods</h3>
<p>Viral DNA from a BoHV6-positive cow was amplified by qPCR and the resulting fragments of the <italic>gB</italic> and <italic>gH</italic> genes encoding glycoproteins B and H (gB and gH) were cloned into the pLATE52 vector to express recombinant gB (rgB) and gH (rgH) in Rosetta (DE3) <italic>E. coli</italic>. The expressed recombinant proteins were used as antigens in the developed ELISA.</p>
</sec>
<sec>
<h3>Results</h3>
<p>The proteins expressed had the expected molecular weight. A total of 143 sera were examined, and 141 of them were positive, according to the chosen cut-off values of 9% and 10% for the sample-to-positive ratios of the rgB and rgH antigens, respectively.</p>
</sec>
<sec>
<h3>Conclusion</h3>
<p>The rgB and rgH recombinant antigens of BoHV6 were successfully expressed in <italic>E. coli</italic> and successfully used in a newly developed ELISA.</p>
</sec>
</abstract>

Introduction
Bovine herpesvirus 6 (BoHV6) belongs to the Herpesviridae family, Gammaherpesvirinae subfamily and Macavirus genus. It is common in cattle, but was also detected in American bison (Bison bison) and water buffalo (Bubalus bubalis). The aim of the experiment was to develop an ELISA for serological examination of cattle sera for the presence of anti-BoHV6 specific antibodies.


Material and Methods
Viral DNA from a BoHV6-positive cow was amplified by qPCR and the resulting fragments of the gB and gH genes encoding glycoproteins B and H (gB and gH) were cloned into the pLATE52 vector to express recombinant gB (rgB) and gH (rgH) in Rosetta (DE3) E. coli. The expressed recombinant proteins were used as antigens in the developed ELISA.


Results
The proteins expressed had the expected molecular weight. A total of 143 sera were examined, and 141 of them were positive, according to the chosen cut-off values of 9% and 10% for the sample-to-positive ratios of the rgB and rgH antigens, respectively.


Conclusion
The rgB and rgH recombinant antigens of BoHV6 were successfully expressed in E. coli and successfully used in a newly developed ELISA.

Development of a recombinant protein-based ELISA for detection of antibodies against bovine herpesvirus 6 (BoHV6)

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction
Bovine herpesvirus 6 (BoHV6) belongs to the Herpesviridae family, Gammaherpesvirinae subfamily and Macavirus genus. It is common in cattle, but...

Development of a recombinant protein-based ELISA for detection of antibodies against bovine herpesvirus 6 (BoHV6)

Pubblicato online: 19 dic 2023

Pagine: 509 - 515

Ricevuto: 08 ott 2023

Accettato: 05 dic 2023

DOI: https://doi.org/10.2478/jvetres-2023-0069

Parole chiave
BoHV6, recombinant glycoprotein B (rgB), rgH, protein expression, ELISA

© 2023 Piotr Kubiś et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Development of a recombinant protein-based ELISA for detection of antibodies against bovine herpesvirus 6 (BoHV6)

Pubblicato online: 19 dic 2023

Pagine: 509 - 515

Ricevuto: 08 ott 2023

Accettato: 05 dic 2023

DOI: https://doi.org/10.2478/jvetres-2023-0069

Parole chiaveBoHV6, recombinant glycoprotein B (rgB), rgH, protein expression, ELISA

© 2023 Piotr Kubiś et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Parole chiave
BoHV6, recombinant glycoprotein B (rgB), rgH, protein expression, ELISA