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Effects of long-term administration of various dietary prebiotic supplements on the growth, immune cell activity and digestive tract histology of juvenile vimba (Vimba vimba)

Barbara Kazuń
Barbara Kazuń
, 
Krzysztof Kazuń
Krzysztof Kazuń
, 
Joanna Małaczewska
Joanna Małaczewska
, 
Rafał Kamiński
Rafał Kamiński
, 
Dobrochna Adamek-Urbańska
Dobrochna Adamek-Urbańska
, 
Justyna Sikorska
Justyna Sikorska
, 
Jacek Wolnicki
Jacek Wolnicki
 e 
Hubert Szudrowicz
Hubert Szudrowicz
   | 16 giu 2023
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Pubblicato online: 16 giu 2023
Pagine: 233 - 241
Ricevuto: 20 gen 2023
Accettato: 22 mag 2023
DOI: https://doi.org/10.2478/jvetres-2023-0033
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© 2023 Barbara Kazuń et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1.

Prebiotic effects after 55 days’ administration on the activity of vimba head-kidney immune cells: respiratory burst activity (A), potential killing activity (B), proliferation of T cells stimulated by concanavalin A (C) and proliferation of B cells stimulated by liposaccharide from E. coli (D). BS – 0.02% Leiber Beta-S–supplemented; MB – 0.20% Leiber Biolex MB40–supplemented; CE – 0.30% Leiber CeFi pro–supplemented; IN – 1.00% BENEO-Orafti Inulin Orafti GR–supplemented. All data are expressed as means ± standard deviation (SD); n = 6. Asterisks refer to statistically significant differences between Control and treatment groups: * P < 0.05, ** P < 0.01
Prebiotic effects after 55 days’ administration on the activity of vimba head-kidney immune cells: respiratory burst activity (A), potential killing activity (B), proliferation of T cells stimulated by concanavalin A (C) and proliferation of B cells stimulated by liposaccharide from E. coli (D). BS – 0.02% Leiber Beta-S–supplemented; MB – 0.20% Leiber Biolex MB40–supplemented; CE – 0.30% Leiber CeFi pro–supplemented; IN – 1.00% BENEO-Orafti Inulin Orafti GR–supplemented. All data are expressed as means ± standard deviation (SD); n = 6. Asterisks refer to statistically significant differences between Control and treatment groups: * P < 0.05, ** P < 0.01

Fig. 2.

Histological structure of vimba posterior intestine in Control (A), BS (B), MB (C), CE (D) and IN (E) groups with PAS-positive, magenta stained absorptive vacuoles in the supranuclear cytoplasm of mucosa (arrow) and intense blue acidic mucous cells. BS – 0.02% Leiber Beta-S–supplemented; MB – 0.20% Leiber MB40–supplemented; CE – 0.30% Leiber CeFi pro–supplemented; IN – 1.00% BENEO-Orafti Inulin Orafti GR–supplemented
Histological structure of vimba posterior intestine in Control (A), BS (B), MB (C), CE (D) and IN (E) groups with PAS-positive, magenta stained absorptive vacuoles in the supranuclear cytoplasm of mucosa (arrow) and intense blue acidic mucous cells. BS – 0.02% Leiber Beta-S–supplemented; MB – 0.20% Leiber MB40–supplemented; CE – 0.30% Leiber CeFi pro–supplemented; IN – 1.00% BENEO-Orafti Inulin Orafti GR–supplemented

Comparison of the final results for fish size, growth rate and efficiency of feed utilisation for vimba (Vimba vimba) fed different experimental prebiotic-supplemented diets for 55 days (Dunnett’s test, n = 3)

Experimental group
Parameter Control BS MB CE IN
mean ± SD mean ± SD P mean ± SD P mean ± SD P mean ± SD P
BW (g) 3.11 ± 0.09 2.74 ± 0.28 0.025 3.04 ± 0.06 0.930 3.00 ± 0.02 0.708 2.96 ± 0.06 0.543
TL (mm) 74.5 ± 0.80 71.1 ± 2.50 0.025 74.2 ± 0.70 0.989 73.3 ± 0.80 0.598 73.4 ± 0.50 0.698
K 0.75 ± 0.00 0.76 ± 0.00 0.526 0.74 ± 0.01 0.877 0.76 ± 0.02 0.499 0.74 ± 0.01 0.999
RGR (%/d) 3.47 ± 0.06 3.23 ± 0.20 0.036 3.43 ± 0.04 0.960 3.41 ± 0.01 0.808 3.39 ± 0.04 0.665
ITL (mm/d) 0.57 ± 0.01 0.51 ± 0.04 0.020 0.56 ± 0.01 0.924 0.55 ± 0.02 0.509 0.54 ± 0.01 0.285
FCR 0.82 ± 0.03 0.97 ± 0.13 0.047 0.84 ± 0.02 0.977 0.86 ± 0.01 0.871 0.87 ± 0.02 0.753

Comparison of histomorphometric analysis of the anterior and posterior intestines and livers in vimba fed different prebiotic-supplemented experimental diets for 55 days (Dunnett’s test, n = 6)

Experimental group
Parameter Control BS MB CE IN
mean ± SD mean ± SD P mean ± SD P mean ± SD P mean ± SD P
Anterior intestine fold height (μm) 398 ± 40 368 ± 28 0.708 396 ± 8 0.999 399 ± 36 0.990 360 ± 15 0.580
Anterior enterocyte height (μm) 38.2 ± 3.6 42.3 ± 2.5 0.232 41.2 ± 3.9 0.368 44.9 ± 4.3 0.025 34.2 ± 0.6 0.557
Anterior intestine width (μm) 94.2 ± 6.2 100.6 ± 4.8 0.471 90.0 ± 6.4 1.000 104.8 ± 11.9 0.134 90.2 ± 10.3 0.993
Posterior intestine fold height (μm) 330 ± 40 284 ± 32 0.103 335 ± 72 0.168 322 ± 37 0.706 316 ± 59 0.155
Posterior enterocyte height (μm) 41.4 ± 2.8 54.0 ± 22.7 0.130 41.4 ± 9.9 0.920 47.1 ± 6.3 0.983 40.2 ± 6.7 0.894
Posterior intestine width (μm) 96.5 ± 9.6 92.5 ± 12.8 0.992 100.9 ± 14.6 0.466 103.9 ± 11.5 0.807 94.7 ± 6.3 0.967
Hepatocyte area (μm2) 120 ± 10 133 ± 21 0.257 122 ± 10 0.986 109 ± 8 0.434 118 ± 10 0.995
Hepatonucleus area (μm2) 20.5 ± 1.1 21.9 ± 1.9 0.300 20.3 ± 1.7 1.000 19.5 ± 1.0 0.601 20.1 ± 1.6 0.983
Hepatonuclear index (%) 17.8 ± 1.2 17.4 ± 1.7 0.969 17.3 ± 1.6 0.932 18.6 ± 1.8 0.791 17.8 ± 1.2 1.000

Prebiotic supplements used in the experiment

Group Prebiotic name Producer Brief characteristics Dosage
BS Leiber Beta-S Leiber GmbH, Germany High-purity 1.3-1.6-beta-D-glucan molecules from the cell wall of Saccharomyces cerevisiae 0.02%
MB Biolex MB40 Leiber GmbH, Germany The cell walls of S. cerevisiae 0.20%
CE CeFi pro Leiber GmbH, Germany The cell contents and the cell walls of S. cerevisiae 0.30%
IN Inulin Orafti GR BENEO-Orafti SA, Belgium 93.4% inulin and 6.6% glucose+fructose+sucrose in dry matter 1.00%
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