P-values |
||||||||
---|---|---|---|---|---|---|---|---|
Virus | State of health | Serum mean ± SD | ELISA cut-off | Milk mean ± SD | ELISA cut-off | Sample | State of health | Sample × state of health |
Healthy | 90.24±3.74a,A | 80.66±6.99b,A | ||||||
BVDV | Mastitic | 94.71±1.39a,B | ≥35% | 93.76±2.87b,B | ≥0.3 | <0.001 | 0.003 | 0.156 |
Healthy | 90.24±3.74a,A | 80.66±6.99b,A | ||||||
BoHV-1 | Mastitic | 94.71±1.39a,B | ≥35% | 93.76±2.87b,B | ≥25% | <0.001 | <0.001 | <0.001 |
Virus | Target gene/amplicon size (bp) | Primers | Sequence (5′–3′) | Reference |
---|---|---|---|---|
BoHV-1 | PF1 | CGGCCACGACGCTGACGA | (15) | |
PF2 | CGCCGCCGAGTACTACCC | |||
BoHV-4 | gB1 | CCCTTCTTTACCACCACCTACA | (29) | |
gB2 | TGCCATAGCAGAGAAACAATGA |
Sample |
|||||
---|---|---|---|---|---|
Serum | Milk | ELISA cut-off | P-value | ||
BoHV-4 positivity rate (%) | Mean ± SD | 110.85 ± 34.39 | 145.64 ± 51.75 | ≥30% | <0.001 |
BoHV-4 positivity degree* | Mean ± SD | 3.15 ± 1.05 | 3.97 ± 1.08 | ||
Median (min–max) | 3 (1–5) | 4 (2–5) | <0.001 |