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Assessment of tuberculosis biomarkers in paratuberculosis-infected cattle

Laura I. Klepp

Laura I. Klepp

  • Institute of Agrobiotechnology and Molecular Biology (IABIMO), INTA-CONICETHurlingham, Argentina
  • Institute of Biotechnology, National Institute of Agricultural TechnologyHurlingham, Argentina
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Klepp, Laura I.
, 
María A. Colombatti

María A. Colombatti

  • Institute of Agrobiotechnology and Molecular Biology (IABIMO), INTA-CONICETHurlingham, Argentina
  • Institute of Biotechnology, National Institute of Agricultural TechnologyHurlingham, Argentina
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Colombatti, María A.
, 
Roberto D. Moyano

Roberto D. Moyano

  • Institute of Agrobiotechnology and Molecular Biology (IABIMO), INTA-CONICETHurlingham, Argentina
  • Institute of Biotechnology, National Institute of Agricultural TechnologyHurlingham, Argentina
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Moyano, Roberto D.
, 
María I. Romano

María I. Romano

  • Institute of Agrobiotechnology and Molecular Biology (IABIMO), INTA-CONICETHurlingham, Argentina
  • Institute of Biotechnology, National Institute of Agricultural TechnologyHurlingham, Argentina
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Romano, María I.
, 
Tadej Malovrh

Tadej Malovrh

Institute for Microbiology and Parasitology, Veterinary Faculty, University of LjubljanaLjubljana, Slovenia
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Malovrh, Tadej
, 
Matjaž Ocepek

Matjaž Ocepek

Institute for Microbiology and Parasitology, Veterinary Faculty, University of LjubljanaLjubljana, Slovenia
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Ocepek, Matjaž
, 
Federico C. Blanco

Federico C. Blanco

  • Institute of Agrobiotechnology and Molecular Biology (IABIMO), INTA-CONICETHurlingham, Argentina
  • Institute of Biotechnology, National Institute of Agricultural TechnologyHurlingham, Argentina
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Blanco, Federico C.
 e 
Fabiana Bigi

Fabiana Bigi

  • Institute of Agrobiotechnology and Molecular Biology (IABIMO), INTA-CONICETHurlingham, Argentina
  • Institute of Biotechnology, National Institute of Agricultural TechnologyHurlingham, Argentina
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Bigi, Fabiana
  
09 mar 2023
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Pubblicato online: 09 mar 2023
Pagine: 55 - 60
Ricevuto: 27 lug 2022
Accettato: 10 feb 2023
DOI: https://doi.org/10.2478/jvetres-2023-0007
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© 2023 L.I. Klepp et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction

Mycobacterium bovis and Mycobacterium avium subsp. paratuberculosis, respectively the causative agents of bovine tuberculosis (bTB) and bovine paratuberculosis (PTB), share a high number of antigenic proteins. This characteristics makes the differential diagnosis of the diseases difficult. The interferon gamma (IFN-γ), C-X-C motif chemokine ligand 10 (CXCL10), matrix metallopeptidase 9 (MMP9), interleukin 22 (IL-22) and thrombospondin 1 (THBS1) bovine genes have already been shown to be accurate transcriptional biomarkers of bTB. In order to improve the diagnosis of bTB and PTB, in the present study we evaluated the risk of false positivity of these bTB biomarkers in cattle with PTB.

Material and Methods

The transcription of these genes was studied in 13 PTB-infected cattle, using Mycobacterium avium subsp. paratuberculosis (MAP)-stimulated peripheral blood mononuclear cells (PBMC).

Results

Overall, the levels of IFN-γ, CXCL10, MMP9 and IL-22 transcripts in MAP-stimulated PBMC failed to differentiate animals with PTB from healthy animals. However, as bTB-afflicted cattle do, the MAP-infected group also displayed a lower level of THBS1 transcription than the non-infected animals.

Conclusion

The results of this study add new specificity attributes to the levels of transcription of IFN-γ, CXCL10, MMP9 and IL-22 as biomarkers for bTB.
Lingua:
Inglese
Frequenza di pubblicazione:
4 volte all'anno
Argomenti della rivista:
Scienze biologiche, Biologia molecolare, Microbiologia e virologia, Scienze della vita, altro, Medicina, Medicina veterinaria
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