Pubblicato online: 18 dic 2022
Pagine: 549 - 557
Ricevuto: 19 ott 2022
Accettato: 06 dic 2022
DOI: https://doi.org/10.2478/jvetres-2022-0070
Parole chiave
© 2022 M. Szymańska-Czerwińska et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Introduction
The intracellular bacterium
Material and Methods
A total of 2,180 sera samples from 801 cattle herds in all Polish voivodeships were tested by ELISA for the presence of specific antibodies. Milk samples were obtained from seropositive cows in 133 herds as part of a separate study. The milk samples were examined by ELISA and real-time PCR tests.
Results
Seroprevalence at the animal level was 7.06% and true positive seroprevalence was 6.0% (95% confidence interval (CI) 1.1–9.4). Seroprevalence at the herd level was estimated at 11.1% and true positive seroprevalence was 10.5% (95% CI 3.2–15.8). Shedding of the pathogen in milk was detected by real-time PCR in 33 out of 133 tested herds (24.81%, 95% CI 17.74–33.04%) and the presence of