<abstract xmlns="http://www.w3.org/1999/xhtml">
<sec id="j_jvetres-2022-0019_s_005">
<h3>Introduction</h3>
<p>Dogs with chronic kidney disease (CKD) may have alterations in the glomerular filtration barrier, including podocyte loss. Detection of podocyte mRNA in urine could be useful for assessing podocyturia in dogs with kidney disease. The objective of this study was to evaluate the presence of nephrin mRNA (<italic>NPHS1</italic>) and podocin mRNA (<italic>NPHS2</italic>) in urine sediments of dogs with naturally occurring CKD and healthy dogs.</p>
</sec>
<sec id="j_jvetres-2022-0019_s_006">
<h3>Material and Methods</h3>
<p>Twenty-four dogs, 14 with CKD and 10 as healthy controls, underwent clinical evaluation. The dogs with CKD were divided into two groups, according to the International Renal Interest Society criteria: stage 1 or 2 CKD (n = 5) and stage 3 or 4 CKD (n = 9). Urine was collected by catheterisation or free catch and RNA isolation from the urine sediments was optimised using glycogen as a co-precipitant. Detection of <italic>NPHS1</italic> and <italic>NPHS2</italic> in the sediment samples was performed using quantitative real-time PCR.</p>
</sec>
<sec id="j_jvetres-2022-0019_s_007">
<h3>Results</h3>
<p>Both types of mRNA were detected in samples from all groups, but the percentages of detection were higher in the group of dogs with stage 1 or 2 CKD and lower in the group of dogs with stage 3 or 4 disease.</p>
</sec>
<sec id="j_jvetres-2022-0019_s_008">
<h3>Conclusion</h3>
<p>Physiological podocyturia was observed in healthy dogs, and the results suggest differential podocyturia in dogs with CKD, according to the stage of the disease, <italic>i.e</italic>. an increase in podocyturia in dogs at stage 1 or 2 and a reduction in podocyturia in dogs at stage 3 or 4.</p>
</sec>
</abstract>

Introduction
Dogs with chronic kidney disease (CKD) may have alterations in the glomerular filtration barrier, including podocyte loss. Detection of podocyte mRNA in urine could be useful for assessing podocyturia in dogs with kidney disease. The objective of this study was to evaluate the presence of nephrin mRNA (NPHS1) and podocin mRNA (NPHS2) in urine sediments of dogs with naturally occurring CKD and healthy dogs.


Material and Methods
Twenty-four dogs, 14 with CKD and 10 as healthy controls, underwent clinical evaluation. The dogs with CKD were divided into two groups, according to the International Renal Interest Society criteria: stage 1 or 2 CKD (n = 5) and stage 3 or 4 CKD (n = 9). Urine was collected by catheterisation or free catch and RNA isolation from the urine sediments was optimised using glycogen as a co-precipitant. Detection of NPHS1 and NPHS2 in the sediment samples was performed using quantitative real-time PCR.


Results
Both types of mRNA were detected in samples from all groups, but the percentages of detection were higher in the group of dogs with stage 1 or 2 CKD and lower in the group of dogs with stage 3 or 4 disease.


Conclusion
Physiological podocyturia was observed in healthy dogs, and the results suggest differential podocyturia in dogs with CKD, according to the stage of the disease, i.e. an increase in podocyturia in dogs at stage 1 or 2 and a reduction in podocyturia in dogs at stage 3 or 4.

Nephrin and podocin mRNA detection in urine sediment of dogs with chronic kidney disease: preliminary observations

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction
Dogs with chronic kidney disease (CKD) may have alterations in the glomerular filtration barrier, including podocyte loss. Detection of podocyte...

Nephrin and podocin mRNA detection in urine sediment of dogs with chronic kidney disease: preliminary observations

Pubblicato online: 22 apr 2022

Pagine: 281 - 288

Ricevuto: 27 ott 2021

Accettato: 29 mar 2022

DOI: https://doi.org/10.2478/jvetres-2022-0019

Parole chiave
dog, podocyturia, quantitative real-time PCR

© 2022 C. de Souza et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Nephrin and podocin mRNA detection in urine sediment of dogs with chronic kidney disease: preliminary observations

Pubblicato online: 22 apr 2022

Pagine: 281 - 288

Ricevuto: 27 ott 2021

Accettato: 29 mar 2022

DOI: https://doi.org/10.2478/jvetres-2022-0019

Parole chiavedog, podocyturia, quantitative real-time PCR

© 2022 C. de Souza et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Parole chiave
dog, podocyturia, quantitative real-time PCR