Genetic and pathogenic characterisation of a virulent Akabane virus isolated from goats in Yunnan, China
Pubblicato online: 10 mar 2022
Pagine: 35 - 42
Ricevuto: 02 giu 2021
Accettato: 01 feb 2022
DOI: https://doi.org/10.2478/jvetres-2022-0007
Parole chiave
© 2022 H.F. Gao et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Introduction
Akabane virus (AKAV) has been detected in a variety of host species in China, but there are only limited records of its occurrence in goats. However, more attention needs to be paid to understanding the diversity of viruses in this species. The aim of the study was to explore the genotype characteristics and variation trend of AKAV and their relationship with virulence in Yunnan, China.
Material and Methods
Blood samples were collected from goats during routine surveillance of goat diseases in Yunnan province in 2019. The AKAV CX-01 strain was isolated using BHK-21 cells. To understand pathogenicity, the virus was intraperitoneally (IP) and intracerebrally (IC) inoculated into suckling mice and tissue samples were subsequently analysed histopathologically and immunohistochemically.
Results
Akabane virus CX-01 strain induced encephalitis and impairment of the central nervous system with fatal consequences. Phylogenetic analysis based on the ORF sequences of the small segments indicated that the AKAV isolate used was most closely related to the GD18134/2018 Chinese midge and bovine NM BS/1strains, while phylogenetic analysis based on the medium segments showed a close relationship between CX-01 and the Chinese GLXCH01 strain.
Conclusion
The CX-01 isolate was related to AKAV genogroup Ia and probably originated from a recombination of different strains.