Comparison of microscopy, card agglutination test for Trypanosoma evansi , and real-time PCR in the diagnosis of trypanosomosis in dromedary camels of the Abu Dhabi Emirate, UAE
Pubblicato online: 25 mar 2022
Pagine: 125 - 129
Ricevuto: 01 lug 2021
Accettato: 03 gen 2022
DOI: https://doi.org/10.2478/jvetres-2022-0002
Parole chiave
© 2022 S. Habeeba et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Introduction
Trypanosomosis is an important disease of dromedary camels caused by the pathogenic protozoan
Material and Methods
Whole blood and serum samples collected from 77 dromedary camels of Abu Dhabi, United Arab Emirates, were analysed with the test methods stated. Statistical analysis was done using McNemar’s chi-squared test, and Cohen’s kappa index (κ) was calculated.
Results
We obtained results with positivity of 18% (14/77) by microscopy, 22% by CATT (17/77) and 60% (46/77) by real-time PCR, with the chain reaction detecting at a respectively three- and two-fold greater rate than the other techniques. Analysis of the data revealed a relative sensitivity of 30.4% and 37.0% for microscopy and CATT, respectively, compared to real-time PCR. The difference between the real-time PCR’s sensitivity and those of the other methods was statistically significant, with X2 values of 30.03 and 20.1, respectively (df = 1 and P = 0.05 in both cases). Agreement of microscopy results with those of with CATT was good (κ = 0.72; 95% CI = 0.62–0.82). Cohen’s kappa index showed fair agreement of real-time PCR with microscopy (κ = 0.26; 95% CI = 0.16–0.36) whereas it was in poor agreement with CATT (κ = 0.09; 95% CI = 0.02–0.15).
Conclusion
Real-time PCR was found to be more sensitive than microscopy and CATT.