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Prevalence of Toxoplasma gondii in retail fresh meat products from free-range chickens in Spain

María Jesús Gracia Salinas

María Jesús Gracia Salinas

Instituto Agroalimentario de Aragón - IA2 (Universidad de Zaragoza - CITA), Veterinary FacultyZaragoza, Spain
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Salinas, María Jesús Gracia
, 
Cristina Escolano Campos

Cristina Escolano Campos

Instituto Agroalimentario de Aragón - IA2 (Universidad de Zaragoza - CITA), Veterinary FacultyZaragoza, Spain
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Campos, Cristina Escolano
, 
María Paz Peris Peris

María Paz Peris Peris

Instituto Agroalimentario de Aragón - IA2 (Universidad de Zaragoza - CITA), Veterinary FacultyZaragoza, Spain
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Peris, María Paz Peris
 e 
Nabil Halaihel Kassab

Nabil Halaihel Kassab

Instituto Agroalimentario de Aragón - IA2 (Universidad de Zaragoza - CITA), Veterinary FacultyZaragoza, Spain
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Kassab, Nabil Halaihel
  
06 ott 2021
Prevalence of Toxoplasma gondii in retail fresh meat products from free-range chickens in Spain's Cover Image
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Categoria dell'articolo: Review article
Pubblicato online: 06 ott 2021
Pagine: 457 - 461
Ricevuto: 14 mar 2021
Accettato: 15 set 2021
DOI: https://doi.org/10.2478/jvetres-2021-0052
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© 2021 M.J. Gracia Salinas et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction

Toxoplasma gondii is one of the most prevalent zoonotic protozoan parasites worldwide and affects the vast majority of warm-blooded animal species, including humans. Postnatal infection in humans occurs through the ingestion of sporulated T. gondii oocysts or via the oral intake of parasite tissue cysts during the consumption of raw or undercooked meat. In this regard, given their high exposure to oocysts, chickens (Gallus domesticus) raised on the ground constitute a potential source of T. gondii.

Material and Methods

For the first time in Spain, a survey was undertaken in commercial retail free-range poultry. A total of 50 thighs from different animals were analysed. The samples were homogenised and an acid pepsin digestion procedure was applied prior to molecular analysis. Toxoplasma gondii DNA was isolated from meat by qPCR. Two sets of primers were used for DNA amplification targeting the specific sequence of a 529 bp repeat element and another set of primers was utilised for the surface antigen protein-1 gene.

Results

DNA extracted from 5 out of 50 tissue samples was positive for both genes by qPCR amplification.

Conclusion

The 10% prevalence of Toxoplasma infection found in commercial free-range chickens raises public health issues.
Lingua:
Inglese
Frequenza di pubblicazione:
4 volte all'anno
Argomenti della rivista:
Scienze biologiche, Biologia molecolare, Microbiologia e virologia, Scienze della vita, altro, Medicina, Medicina veterinaria
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