Molecular identification and antifungal susceptibility patterns of dermatophytes isolated from companion animals with clinical symptoms of dermatophytosis
, , , e | 23 apr 2021
INFORMAZIONI SU QUESTO ARTICOLO
Pubblicato online: 23 apr 2021
Pagine: 175 - 182
Ricevuto: 11 mag 2019
Accettato: 24 mar 2021
DOI: https://doi.org/10.2478/jvetres-2021-0020
Parole chiave
© 2021 F. Katiraee et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Fig 1
Results of direct microscopic examinations versus those of cultures
| Test results | Animal | Total (n=308) | ||
|---|---|---|---|---|
| Dog (n=134) | Cat (n=174) | |||
| Microscopy | Positive |
62 (46.2%) |
64 (38.4%) |
126 (40.9%) |
| Negative | 72 (53.8%) | 110 (61.6%) | 182 (59.1%) | |
| Culture | Positive |
62 (46.2%) |
68 (39.1%) |
130 (42.2%) |
| Negative | 72 (53.8%) | 106 (60.9%) | 178 (57.8%) | |
Profiles of animals with suspected cases of dermatophytosis
| Animal | Total (n=130) | |||
|---|---|---|---|---|
| Dog (n=62) | Cat (n=68) | |||
| Age | <1 year old |
36 (58.1%) |
44 (64.7%) |
80 (61.5%) |
| Sex | Female |
26 (41.9%) |
46 (67.6%) |
72 (55.4%) |
| Habitat | Indoor |
50 (80.6%) |
34 (50%) |
84 (64.6%) |
| Disease in owner | Yes |
8 (12.9%) |
18 (26.5%) |
26 (25%) |
| Previous antifungal therapy for cutaneous lesions | Yes |
4 (6.5%) |
6 (8.8%) |
10 (7.7%) |
In vitro antifungal susceptibilities of eight antifungal drugs against 130 clinical isolates of dermatophytes from dogs and cats with dermatophytosis
| Dermatophyte species | Drug | MIC range (μg/mL) | GM (μg/mL) | MIC(μg/mL) 50 | MIC(μg/mL) 90 |
|---|---|---|---|---|---|
| Caspofungin | 0.001–0.008 | 0.001 | 0.001 | 0.002 | |
| Ketoconazole | 0.032–8 | 0.064 | 0.032 | 0.125 | |
| Miconazole | 0.032–0.5 | 0.12 | 0.250 | 0.250 | |
| Itraconazole | 0.002–2 | 0.06 | 0.125 | 0.25 | |
| (n=102) | Griseofulvin | 0.125–8 | 0.6 | 1 | 1 |
| Clotrimazole | 0.016–16 | 0.12 | 1 | 2 | |
| Fluconazole | 0.128–32 | 2.1 | 4 | 8 | |
| Terbinafine | 0.008–2 | 0.064 | 0.125 | 0.25 | |
| Caspofungin | 0.001–0.016 | 0.0025 | 0.002 | 0.004 | |
| Ketoconazole | 0.016–0.128 | 0.026 | 0.016 | 0.032 | |
| Miconazole | 0.064–0.5 | 0.2 | 0.128 | 0.5 | |
| Itraconazole | 0.002–1 | 0.09 | 0.125 | 0.25 | |
| Griseofulvin | 0.128–2 | 0.25 | 0.250 | 1.0 | |
| Clotrimazole | 0.5–8 | 0.9 | 0.5 | 4 | |
| Fluconazole | 0.5–4 | 1.41 | 1 | 4 | |
| Terbinafine | 0.016–0.125 | 0.026 | 0.064 | 0.064 | |
| Caspofungin | 0.001–0.004 | 0.0019 | 0.002 | 0.008 | |
| Ketoconazole | 0.016–0.064 | 0.026 | 0.016 | 0.064 | |
| Miconazole | 0.064–0.5 | 0.15 | 0.125 | 0.5 | |
| Itraconazole | 0.002–0.5 | 0.07 | 0.125 | 0.25 | |
| (n=14) | Griseofulvin | 0.064–2 | 0.23 | 0.125 | 1.0 |
| Clotrimazole | 0.25–4 | 0.82 | 0.5 | 4 | |
| Fluconazole | 0.25–4 | 1.1 | 1 | 2 | |
| Terbinafine | 0.016–0.064 | 0.039 | 0.016 | 0.064 | |
Results of ITS sequence analysis of canine and feline dermatophyte samples
| Molecular results | Animal | Total (n=130) | ||
|---|---|---|---|---|
| Dog (n=62) | Cat (n=68) | |||
| 46 (74.3%) | 56 (82.3%) | 102 (78.4%) | ||
| ITS sequenced analysis | 10 (16%) | 4 (5.9%) | 14 (10.8%) | |
| 6 (9.7%) | 8 (11.8%) | 14 (10.8%) | ||
Fungal strains isolated from animals with skin lesions suspected of being dermatophytes
| Genus | Fungal species | Dog | Cat | Total |
| 46 |
56 |
102 |
||
| 22 | 26 | 48 | ||
| 2 | 2 | 4 | ||
| 1 | 3 | 4 | ||
| 3 | 0 | 3 | ||
| 1 | 0 | 1 | ||
| 0 | 2 | 2 |