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Phytoncides in the prevention and therapy of blackhead disease and their effect on the turkey immune system

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29 gen 2021
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Fig. 1

Detection of H. meleagridis by PCR assayK− – negative control; K+ – positive control (574 bp); 1, 2 – DNA samples isolated from liver; 3 – DNA sample isolated from caecum; M – molecular size marker (100–1000 bp)
Detection of H. meleagridis by PCR assayK− – negative control; K+ – positive control (574 bp); 1, 2 – DNA samples isolated from liver; 3 – DNA sample isolated from caecum; M – molecular size marker (100–1000 bp)

Mean values of production parameters and the mortality and culling rate in the laying production period (week 32 to week 56 of life) in a reproductive flock of turkeys treated for histomonosis diagnosed in the rearing period

Parameter Turkey house
P-1 P-2 P-3
Eggs/hen 112 117 119
Mortality and culling (%) 6.1 2.7 3.4
P-1, P-2, and P-3
Fertility (%) 97
Hatchability (%) 85

Mean percentage of CD4+, CD8+, and CD4+CD8+ T lymphocytes and IgM+ B cells ± SD in blood and spleen of turkeys from experiment I (n = 7)

Blood Spleen
Group CD4+ CD8+ CD4+CD8+ IgM+ CD4+ CD8+ CD4+CD8+ IgM+
A1 16.95a ± 2.44 2.20 ± 0.38 0.57 ± 0.08 10.86 ± 0.99 34.80 ± 6.80 21.35 ± 6.14 1.32 ± 0.36 17.47a ± 2.21
A2 23.68b ± 4.88 2.19 ± 0.70 0.71 ± 0.17 11.55 ± 1.15 37.27 ± 7.38 25.15 ± 10.00 1.46 ± 0.37 20.85ab ± 2.10
A3 18.53ab ± 6.05 2.17 ± 0.42 0.57 ± 0.11 11.54 ± 0.98 33.15 ± 3.19 28.40 ± 8.49 1.55 ± 0.54 22.10b ± 3.32

Mean geometric titre of post-vaccination anti-ORT IgY antibodies in blood serum and %CV of turkeys from experiment II (n = 23)

Day of vaccination 3 weeks post vaccination
Group ORT (titre) ORT (%CV) ORT (titre) ORT (%CV)
B1 128 137 2,312 64
B2 123 129 2,778 75
B3 125 134 1,567 54

Mean value ± SD of biochemical markers in blood serum of turkeys from experiment I (n = 10)

Group ALT (U/L) AST (U/L) ALP (U/L) CK (U/L) LDH (U/L) TP (g/L)
A1 15.83 ± 1.26 261.67 ± 14.39 2056.67 ± 110.32 1287.90 ± 260.08 942.00 ± 91.42 35.45 ± 2.26
A2 16.00 ± 2.10 250.50 ± 18.97 1768.83 ± 360.05 1450.92 ± 255.59 1002.83 ± 88.23 35.61 ± 1.65
A3 17.33 ± 3.39 275.33 ± 39.26 2311.33 ± 542.02 1934.52 ± 399.42 1034.17 ± 67.31 35.63 ± 4.25
Lingua:
Inglese
Frequenza di pubblicazione:
4 volte all'anno
Argomenti della rivista:
Scienze biologiche, Biologia molecolare, Microbiologia e virologia, Scienze della vita, altro, Medicina, Medicina veterinaria