<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec id="j_jvetres-2021-0005_s_005">
<h3>Introduction</h3>
<p>Foot-and-mouth disease is a highly infectious viral disease affecting all cloven-footed domestic animals. The three foot-and-mouth disease virus (FMDV) serotypes A, O and SAT2 are at present the greatest threat to susceptible animals in Egypt. The aim of the present study was, for the host factors associated with different FMDV infections in cattle during the acute phase, to compare these factors’ influence on the expression of the <italic>IL-10</italic>, <italic>TLR-2</italic>, <italic>TNF-α</italic>, <italic>CXCL10</italic>, <italic>CD48</italic>, <italic>NFATC4</italic> and <italic>IFNG</italic> inflammatory and immune-related genes.</p></sec>
<sec id="j_jvetres-2021-0005_s_006">
<h3>Materials and methods</h3>
<p>Vesicular fluid and epithelium samples were obtained from at least three infected cattle on the same affected farm during three different FMDV outbreaks and were used for serotyping of the virus and for expression analysis of host genes. A two-step RT-PCR was used for diagnosis of the virus with primers specific for each serotype.</p></sec>
<sec id="j_jvetres-2021-0005_s_007">
<h3>Results</h3>
<p>In quantitative PCR analysis, the expression patterns of <italic>TLR-2</italic> and <italic>IFNG</italic> were prominent, while <italic>NFATC4</italic> expression was absent in all FMDV-infected cattle. The highest expression of <italic>CD48</italic> was associated with increased expression of other inflammatory and immune-related genes (<italic>IL-10</italic>, <italic>TLR-2</italic>, <italic>TNF-α</italic> and <italic>IFNG</italic>), which may be an indication of rapid virus clearance.</p></sec>
<sec id="j_jvetres-2021-0005_s_008">
<h3>Conclusion</h3>
<p>The use of vesicular fluid and epithelium for investigation of viral and immune-related gene expression levels in acute FMDV infection is possible. Host-dependent variation in the expression of the studied genes was observed in different FMDV serotype outbreaks.</p></sec>
</abstract>

Introduction
Foot-and-mouth disease is a highly infectious viral disease affecting all cloven-footed domestic animals. The three foot-and-mouth disease virus (FMDV) serotypes A, O and SAT2 are at present the greatest threat to susceptible animals in Egypt. The aim of the present study was, for the host factors associated with different FMDV infections in cattle during the acute phase, to compare these factors’ influence on the expression of the IL-10, TLR-2, TNF-α, CXCL10, CD48, NFATC4 and IFNG inflammatory and immune-related genes.

Materials and methods
Vesicular fluid and epithelium samples were obtained from at least three infected cattle on the same affected farm during three different FMDV outbreaks and were used for serotyping of the virus and for expression analysis of host genes. A two-step RT-PCR was used for diagnosis of the virus with primers specific for each serotype.

Results
In quantitative PCR analysis, the expression patterns of TLR-2 and IFNG were prominent, while NFATC4 expression was absent in all FMDV-infected cattle. The highest expression of CD48 was associated with increased expression of other inflammatory and immune-related genes (IL-10, TLR-2, TNF-α and IFNG), which may be an indication of rapid virus clearance.

Conclusion
The use of vesicular fluid and epithelium for investigation of viral and immune-related gene expression levels in acute FMDV infection is possible. Host-dependent variation in the expression of the studied genes was observed in different FMDV serotype outbreaks.

Comparative expression analysis of inflammatory and immune-related genes in cattle during acute infection with foot-and-mouth disease virus in Egypt

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction
Foot-and-mouth disease is a highly infectious viral disease affecting all cloven-footed domestic animals. The three foot-and-mouth disease virus...

Comparative expression analysis of inflammatory and immune-related genes in cattle during acute infection with foot-and-mouth disease virus in Egypt

Pubblicato online: 26 gen 2021

Pagine: 39 - 44

Ricevuto: 14 lug 2020

Accettato: 29 dic 2020

DOI: https://doi.org/10.2478/jvetres-2021-0005

Parole chiave
FMDV, gene expression, cattle, immune-related genes, serotype

© 2021 A.F. El Nahas et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Comparative expression analysis of inflammatory and immune-related genes in cattle during acute infection with foot-and-mouth disease virus in Egypt

Pubblicato online: 26 gen 2021

Pagine: 39 - 44

Ricevuto: 14 lug 2020

Accettato: 29 dic 2020

DOI: https://doi.org/10.2478/jvetres-2021-0005

Parole chiaveFMDV, gene expression, cattle, immune-related genes, serotype

© 2021 A.F. El Nahas et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Parole chiave
FMDV, gene expression, cattle, immune-related genes, serotype