Detection of white sturgeon iridovirus (WSIV) in wild sturgeons (Actinopterygii: Acipenseriformes: Acipenseridae) in Poland
Pubblicato online: 16 set 2020
Pagine: 363 - 368
Ricevuto: 29 feb 2020
Accettato: 13 ago 2020
DOI: https://doi.org/10.2478/jvetres-2020-0055
Parole chiave
© 2020 P. Hofsoe-Oppermann et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Introduction
White sturgeon iridovirus (WSIV) disease is caused by a virus of the eponymous family and is mostly triggered by stressful environmental conditions,
Material and Methods
A total of 42 samples (spleen, gills, intestine, skin, kidney, and brain) were collected from eight sturgeon (
Results
In gross examination, all fish appeared to be healthy. Neither species showed clinical signs typical of WSIV infection. In the majority of cases, fragments of iridoviral DNA were found using molecular methods in the kidneys, and also in the liver, gills, and skin. The detection rate using ISH was 47.37% and most commonly the brain and kidney tissues were positive. The most efficient of the methods used was real-time PCR, with 100% effectiveness in detection of WSIV DNA.
Conclusion
The study demonstrates the capabilities for WSIV diagnosis available to sturgeon farmers and water administrators, indicating useful methods of adequate sensitivity as well as organs to sample in order to achieve the highest probability of viral detection.