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Genetic characterisation of African swine fever virus in outbreaks in Ha Nam province, Red River Delta Region of Vietnam, and activity of antimicrobial products against virus infection in contaminated feed

Ha Thi Thanh Tran

Ha Thi Thanh Tran

Department of Biochemistry and Immunology, National Institute of Veterinary ResearchHanoi, Vietnam
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Tran, Ha Thi Thanh
, 
Anh Duc Truong

Anh Duc Truong

Department of Biochemistry and Immunology, National Institute of Veterinary ResearchHanoi, Vietnam
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Truong, Anh Duc
, 
Duc Viet Ly

Duc Viet Ly

Department of Biochemistry and Immunology, National Institute of Veterinary ResearchHanoi, Vietnam
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Ly, Duc Viet
, 
Thi Hao Vu

Thi Hao Vu

Department of Biochemistry and Immunology, National Institute of Veterinary ResearchHanoi, Vietnam
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Vu, Thi Hao
, 
Van Tuan Hoang

Van Tuan Hoang

Department of Biochemistry and Immunology, National Institute of Veterinary ResearchHanoi, Vietnam
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Hoang, Van Tuan
, 
Thi Chinh Nguyen

Thi Chinh Nguyen

Department of Biochemistry and Immunology, National Institute of Veterinary ResearchHanoi, Vietnam
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Nguyen, Thi Chinh
, 
Thi Nhu Chu

Thi Nhu Chu

Department of Biochemistry and Immunology, National Institute of Veterinary ResearchHanoi, Vietnam
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Chu, Thi Nhu
, 
Thi Huyen Nguyen

Thi Huyen Nguyen

Department of Biochemistry and Immunology, National Institute of Veterinary ResearchHanoi, Vietnam
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Nguyen, Thi Huyen
, 
Ngoc Thi Pham

Ngoc Thi Pham

Department of Biochemistry and Immunology, National Institute of Veterinary ResearchHanoi, Vietnam
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Pham, Ngoc Thi
, 
Tinh Nguyen

Tinh Nguyen

Kemin Industries (Vietnam) Co. Ltd.Kemin, Vietnam
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Nguyen, Tinh
, 
Andrew G. Yersin

Andrew G. Yersin

Kemin Industries, Inc.Des Moines, USA
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Yersin, Andrew G.
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Hoang Vu Dang

Hoang Vu Dang

Department of Biochemistry and Immunology, National Institute of Veterinary ResearchHanoi, Vietnam
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Dang, Hoang Vu
  
16 giu 2020
Genetic characterisation of African swine fever virus in outbreaks in Ha Nam province, Red River Delta Region of Vietnam, and activity of antimicrobial products against virus infection in contaminated feed's Cover Image
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Pubblicato online: 16 giu 2020
Pagine: 207 - 213
Ricevuto: 11 nov 2019
Accettato: 01 giu 2020
DOI: https://doi.org/10.2478/jvetres-2020-0041
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© 2020 H.T.T. Tran et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Detection of ASFV in a field sample of spleen tissue. A – HAD assay of the spleen homogenate; B– the real-time PCR amplification of the p72 gene
Detection of ASFV in a field sample of spleen tissue. A – HAD assay of the spleen homogenate; B– the real-time PCR amplification of the p72 gene

Fig. 2

Phylogenetic analysis of VN/Pig/HN/19 based on its partial p72 (A) and EP402R gene encoding the CD2v protein (B) genes. Numbers along branches indicate bootstrap values >70% (1,000 replicates). The black triangle indicates the ASFV isolate from this study. Scale bars indicate nucleotide substitutions per site
Phylogenetic analysis of VN/Pig/HN/19 based on its partial p72 (A) and EP402R gene encoding the CD2v protein (B) genes. Numbers along branches indicate bootstrap values >70% (1,000 replicates). The black triangle indicates the ASFV isolate from this study. Scale bars indicate nucleotide substitutions per site

Fig. 3

(A, B). The combination of feed with the Sal CURB RM E Liquid, Sal CURB F2 Dry or Sal CURB K2 Liquid and 1×105/mL HAD50 of VN/Pig/HN/19 strain one, three, and seven days post inoculation. Values represent mean and standard deviation results from three independent experiments. Significant differences compared to control are denoted by * for P < 0.05 and ** for P < 0.01
(A, B). The combination of feed with the Sal CURB RM E Liquid, Sal CURB F2 Dry or Sal CURB K2 Liquid and 1×105/mL HAD50 of VN/Pig/HN/19 strain one, three, and seven days post inoculation. Values represent mean and standard deviation results from three independent experiments. Significant differences compared to control are denoted by * for P < 0.05 and ** for P < 0.01

Fig. 4

(A, B). The combination of feed with the Sal CURB RM E Liquid, Sal CURB F2 Dry or Sal CURB K2 Liquid and 1×105/mL HAD50 of VN/Pig/HN/19 strain one, three, and seven days post inoculation. Values represent mean and standard deviation results from three independent experiments. Significant differences compared to control are denoted by * for P < 0.05 and ** for P < 0.01
(A, B). The combination of feed with the Sal CURB RM E Liquid, Sal CURB F2 Dry or Sal CURB K2 Liquid and 1×105/mL HAD50 of VN/Pig/HN/19 strain one, three, and seven days post inoculation. Values represent mean and standard deviation results from three independent experiments. Significant differences compared to control are denoted by * for P < 0.05 and ** for P < 0.01
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Inglese
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Scienze biologiche, Biologia molecolare, Microbiologia e virologia, Scienze della vita, altro, Medicina, Medicina veterinaria
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