<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec id="j_jvetres-2020-0011_s_006">
<h3>Introduction</h3>
<p>Leishmaniasis is a zoonotic disease which is caused by protozoan parasites of the genus <italic>Leishmania</italic>. Canids are the most important reservoir of the parasites; however, limited data are available on the species of <italic>Leishmania</italic> prevalent in these animals and their impact on human health. The objective of this study was to estimate the seroprevalence of leishmaniasis in dogs from an inter-Andean region of Colombia during July 2016–July 2017, and to describe the clinical and histopathological features of the disease.</p>
</sec>
<sec id="j_jvetres-2020-0011_s_007">
<h3>Material and Methods</h3>
<p>A total of 155 dogs were subjected to clinical examination and a serological test for detection of antibodies against <italic>Leishmania</italic>. Necropsy was carried out on positive animals and tissue samples were processed by routine histopathology.</p>
</sec>
<sec id="j_jvetres-2020-0011_s_008">
<h3>Results</h3>
<p>Altogether 19 dogs were positive in the serological test, establishing a 12% seroprevalence of <italic>Leishmania</italic>. Clinical examination and necropsy revealed exfoliative and ulcerative dermatitis with haemorrhagic borders on the ears, head, nose, and legs. Histopathology revealed severe multifocal dermatitis with abundant <italic>Leishmania</italic> amastigotes within the cytoplasm of phagocytic cells, depletion of lymphocytes in lymphoid tissues, interstitial pneumonia, and interstitial nephritis. Tissue samples were positive for <italic>Leishmania</italic> by PCR.</p>
</sec>
<sec id="j_jvetres-2020-0011_s_009">
<h3>Conclusion</h3>
<p>The macro- and microscopic changes correlated with the presence of <italic>Leishmania</italic> as established by serological test and PCR.</p>
</sec>
</abstract>

Introduction
Leishmaniasis is a zoonotic disease which is caused by protozoan parasites of the genus Leishmania. Canids are the most important reservoir of the parasites; however, limited data are available on the species of Leishmania prevalent in these animals and their impact on human health. The objective of this study was to estimate the seroprevalence of leishmaniasis in dogs from an inter-Andean region of Colombia during July 2016–July 2017, and to describe the clinical and histopathological features of the disease.


Material and Methods
A total of 155 dogs were subjected to clinical examination and a serological test for detection of antibodies against Leishmania. Necropsy was carried out on positive animals and tissue samples were processed by routine histopathology.


Results
Altogether 19 dogs were positive in the serological test, establishing a 12% seroprevalence of Leishmania. Clinical examination and necropsy revealed exfoliative and ulcerative dermatitis with haemorrhagic borders on the ears, head, nose, and legs. Histopathology revealed severe multifocal dermatitis with abundant Leishmania amastigotes within the cytoplasm of phagocytic cells, depletion of lymphocytes in lymphoid tissues, interstitial pneumonia, and interstitial nephritis. Tissue samples were positive for Leishmania by PCR.


Conclusion
The macro- and microscopic changes correlated with the presence of Leishmania as established by serological test and PCR.

Seroprevalence, clinical, and pathological characteristics of canine leishmaniasis in a central region of Colombia

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction
Leishmaniasis is a zoonotic disease which is caused by protozoan parasites of the genus Leishmania. Canids are the most important reservoir of the...

Seroprevalence, clinical, and pathological characteristics of canine leishmaniasis in a central region of Colombia

Pubblicato online: 14 feb 2020

Pagine: 85 - 94

Ricevuto: 28 mar 2019

Accettato: 03 feb 2020

DOI: https://doi.org/10.2478/jvetres-2020-0011

Parole chiave
antibodies, clinical examination, necropsy, histopathology

© 2020 Y. Picón et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Seroprevalence, clinical, and pathological characteristics of canine leishmaniasis in a central region of Colombia

Pubblicato online: 14 feb 2020

Pagine: 85 - 94

Ricevuto: 28 mar 2019

Accettato: 03 feb 2020

DOI: https://doi.org/10.2478/jvetres-2020-0011

Parole chiaveantibodies, clinical examination, necropsy, histopathology

© 2020 Y. Picón et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Parole chiave
antibodies, clinical examination, necropsy, histopathology