<abstract xmlns="http://www.w3.org/1999/xhtml"><bold>Introduction:</bold><italic>Fasciola hepatica</italic> (liver fluke) is a parasite of great socioeconomic importance. A number of fluke isolates have been identified; however, to date the differences between the immunomodulatory properties of different parasite isolates have not been sufficiently investigated. The aim of this study was to explore differences between the immunomodulatory properties of two <italic>F. hepatica</italic> isolates using unmaturated bovine macrophages.<bold>Material and Methods:</bold> A cell line of bovine macrophages was stimulated with excretory/secretory products released by adult flukes from either a laboratory (<italic>Fh</italic>-WeyES) or wild (<italic>Fh</italic>-WildES) strain and subsequently subjected to microarray and ELISA analyses.<bold>Results:</bold> Both <italic>Fh</italic>-WeyES and <italic>Fh</italic>-WildES dampened the release of interleukin-10 by bovine macrophages, but only <italic>Fh</italic>-WildES dampened the release of proinflammatory tumour necrosis factor-α. Microarray analysis revealed that <italic>Fh</italic>-WildES down- and upregulated 90 and 18 genes, respectively, when compared to <italic>Fh</italic>-WeyES.<bold>Conclusion:</bold> The results indicated different impacts of the isolates on macrophages. A number of researchers use flukes obtained from local slaughterhouses for experiments. Our findings may explain some discrepancies between published results arising from parasite strain choice. The findings indicate that consideration should be given to the use of different strains, and open new and currently unexplored avenues in parasitology for controlling the parasite.</abstract>

Introduction:Fasciola hepatica (liver fluke) is a parasite of great socioeconomic importance. A number of fluke isolates have been identified; however, to date the differences between the immunomodulatory properties of different parasite isolates have not been sufficiently investigated. The aim of this study was to explore differences between the immunomodulatory properties of two F. hepatica isolates using unmaturated bovine macrophages.Material and Methods: A cell line of bovine macrophages was stimulated with excretory/secretory products released by adult flukes from either a laboratory (Fh-WeyES) or wild (Fh-WildES) strain and subsequently subjected to microarray and ELISA analyses.Results: Both Fh-WeyES and Fh-WildES dampened the release of interleukin-10 by bovine macrophages, but only Fh-WildES dampened the release of proinflammatory tumour necrosis factor-α. Microarray analysis revealed that Fh-WildES down- and upregulated 90 and 18 genes, respectively, when compared to Fh-WeyES.Conclusion: The results indicated different impacts of the isolates on macrophages. A number of researchers use flukes obtained from local slaughterhouses for experiments. Our findings may explain some discrepancies between published results arising from parasite strain choice. The findings indicate that consideration should be given to the use of different strains, and open new and currently unexplored avenues in parasitology for controlling the parasite.

<italic>Fasciola hepatica</italic> isolates induce different immune responses in unmaturated bovine macrophages

Division of Pharmacology and Toxicology, Department of Preclinical Sciences

Witold Stefański Institute of Parasitology, Polish Academy of Sciences

Division of Parasitology and Parasitic Diseases, Department of Preclinical Sciences, Faculty of Veterinary Medicine

Central Forensic Laboratory of the Police (CFLP)

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

 isolates induce different immune responses in unmaturated bovine macrophages

Introduction:Fasciola hepatica (liver fluke) is a parasite of great socioeconomic importance. A number of fluke isolates have been identified; however, to date...

Fasciola hepatica isolates induce different immune responses in unmaturated bovine macrophages

Pubblicato online: 28 mar 2019

Pagine: 63 - 70

Ricevuto: 13 ago 2018

Accettato: 25 feb 2019

DOI: https://doi.org/10.2478/jvetres-2019-0011

Parole chiave
macrophages, gene expression, immunoregulation, immune modulation

© 2019 Piotr Bąska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fasciola hepatica isolates induce different immune responses in unmaturated bovine macrophages

Pubblicato online: 28 mar 2019

Pagine: 63 - 70

Ricevuto: 13 ago 2018

Accettato: 25 feb 2019

DOI: https://doi.org/10.2478/jvetres-2019-0011

Parole chiavemacrophages, gene expression, immunoregulation, immune modulation

© 2019 Piotr Bąska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Parole chiave
macrophages, gene expression, immunoregulation, immune modulation