<abstract xmlns="http://www.w3.org/1999/xhtml"><bold>Introduction:</bold> Avian reovirus (ARV) infections in poultry populations are reported worldwide. The reovirus belongs to the genus <italic>Orthoreovirus</italic>, family <italic>Reoviridae</italic>. The aim of the study was to evaluate the incidence of ARV infections in the poultry population based on diagnostic tests performed in 2010–2017.<bold>Material and Methods:</bold> Samples of the liver and spleen were collected from sick birds suspected of ARV infection and sent for diagnostics. Isolation was performed in 5–7-day-old SPF chicken embryos infected into the yolk sac with homogenates of internal organs of sick birds. Four primer pairs were used to detect the σNS, σC, σA, and µA ARV RNA gene fragments. A nested PCR was used for the detection of the σNS and σC genes.<bold>Results:</bold> In 2010–2017, ARV infection was found in birds from 81 flocks of broiler chickens and/or layers, 8 flocks of slaughter turkeys, and in 4 hatchery embryos at 17–20 days of incubation. The primers used in RT-PCR and nested PCR did not allow effective detection of ARV RNA in all virus-positive samples.<bold>Conclusion:</bold> The problem of ARV infections in the poultry population in Poland still persist. The primers used for various ARV segments in RT-PCR and nested PCR did not allow effective detection of RNA in the visceral organs of sick birds. The presented results confirm the necessity of using classical diagnostic methods (isolation in chicken embryos, AGID).</abstract>

Introduction: Avian reovirus (ARV) infections in poultry populations are reported worldwide. The reovirus belongs to the genus Orthoreovirus, family Reoviridae. The aim of the study was to evaluate the incidence of ARV infections in the poultry population based on diagnostic tests performed in 2010–2017.Material and Methods: Samples of the liver and spleen were collected from sick birds suspected of ARV infection and sent for diagnostics. Isolation was performed in 5–7-day-old SPF chicken embryos infected into the yolk sac with homogenates of internal organs of sick birds. Four primer pairs were used to detect the σNS, σC, σA, and µA ARV RNA gene fragments. A nested PCR was used for the detection of the σNS and σC genes.Results: In 2010–2017, ARV infection was found in birds from 81 flocks of broiler chickens and/or layers, 8 flocks of slaughter turkeys, and in 4 hatchery embryos at 17–20 days of incubation. The primers used in RT-PCR and nested PCR did not allow effective detection of ARV RNA in all virus-positive samples.Conclusion: The problem of ARV infections in the poultry population in Poland still persist. The primers used for various ARV segments in RT-PCR and nested PCR did not allow effective detection of RNA in the visceral organs of sick birds. The presented results confirm the necessity of using classical diagnostic methods (isolation in chicken embryos, AGID).

Occurrence of reovirus (ARV) infections in poultry flocks in Poland in 2010–2017

Department of Poultry Diseases, National Veterinary Research Institute

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction: Avian reovirus (ARV) infections in poultry populations are reported worldwide. The reovirus belongs to the genus Orthoreovirus, family...

Occurrence of reovirus (ARV) infections in poultry flocks in Poland in 2010–2017

Pubblicato online: 31 dic 2018

Pagine: 421 - 426

Ricevuto: 22 ago 2018

Accettato: 12 dic 2018

DOI: https://doi.org/10.2478/jvetres-2018-0079

Parole chiave
poultry, avian reovirus, isolation, RT-PCR

© 2018 Hanna Czekaj et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Occurrence of reovirus (ARV) infections in poultry flocks in Poland in 2010–2017

Pubblicato online: 31 dic 2018

Pagine: 421 - 426

Ricevuto: 22 ago 2018

Accettato: 12 dic 2018

DOI: https://doi.org/10.2478/jvetres-2018-0079

Parole chiavepoultry, avian reovirus, isolation, RT-PCR

© 2018 Hanna Czekaj et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Parole chiave
poultry, avian reovirus, isolation, RT-PCR