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The Effect of Trifluralin on Post-in vitro Morphogenesis of Five Genotypes of Head Cabbage (Brassica oleraceae var. capitata)

Oushadee A.J. Abeyawardana

Oushadee A.J. Abeyawardana

  • Department of Horticulture, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences PraguePrague-Suchdol, Czech Republic
  • Laboratory of Plant reproduction, Institute of Experimental Botany, Czech Academy of SciencesPrague 1, Czech Republic
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Abeyawardana, Oushadee A.J.
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Iva Viehmannová

Iva Viehmannová

Department of Crop Sciences and Agroforestry, Faculty of Tropical AgriSciences, Czech University of Life Sciences PraguePrague-Suchdol, Czech Republic
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Viehmannová, Iva
 e 
Martin Koudela

Martin Koudela

Department of Horticulture, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences PraguePrague-Suchdol, Czech Republic
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Koudela, Martin
  
03 mag 2021
The Effect of Trifluralin on Post-in vitro Morphogenesis of Five Genotypes of Head Cabbage (Brassica oleraceae var. capitata)'s Cover Image
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Categoria dell'articolo: Short communication
Pubblicato online: 03 mag 2021
Pagine: 47 - 54
Ricevuto: 01 nov 2020
Accettato: 01 mar 2021
DOI: https://doi.org/10.2478/johr-2021-0007
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© 2021 Oushadee A.J. Abeyawardana et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1

Organogenesis of cabbage in in vitro cultures: A – shoot induction from cut ends of treated hypocotyl on shoot induction medium in control (bar = 0.5 cm); B – shoot induction from trifluralin treated callus on shoot induction medium (bar = 0.5 cm); C – green dormant callus from treated calli on shoot induction medium (bar = 0.5 cm); D – germinating seeds treated with trifluralin after seven days on seed germination medium (bar = 1 cm); E – growing calli from treated hypocotyls after 14 days on shoot induction medium (bar = 0.5 cm); F – rooted shootlets derived from treated seeds
Organogenesis of cabbage in in vitro cultures: A – shoot induction from cut ends of treated hypocotyl on shoot induction medium in control (bar = 0.5 cm); B – shoot induction from trifluralin treated callus on shoot induction medium (bar = 0.5 cm); C – green dormant callus from treated calli on shoot induction medium (bar = 0.5 cm); D – germinating seeds treated with trifluralin after seven days on seed germination medium (bar = 1 cm); E – growing calli from treated hypocotyls after 14 days on shoot induction medium (bar = 0.5 cm); F – rooted shootlets derived from treated seeds

Figure 2

Effect of cultivar, trifluralin concentration, treatment condition on in vitro culture of seeds and their interaction with each other: A1, B1, C1 – the effect of different treatment conditions on the rate of seed germination, shoot induction, and root induction respectively; A2, B2, C2 – the effect of different cultivars on the rate of seed germination, shoot induction, and root induction respectively; A3, B3, C3 – the effect of different concentrations of trifluralin on seed germination, shoot induction, and root induction respectively. Vertical bars denote 0.95 confidence intervals
Effect of cultivar, trifluralin concentration, treatment condition on in vitro culture of seeds and their interaction with each other: A1, B1, C1 – the effect of different treatment conditions on the rate of seed germination, shoot induction, and root induction respectively; A2, B2, C2 – the effect of different cultivars on the rate of seed germination, shoot induction, and root induction respectively; A3, B3, C3 – the effect of different concentrations of trifluralin on seed germination, shoot induction, and root induction respectively. Vertical bars denote 0.95 confidence intervals

Results of ANOVA to test the influence of cultivar, trifluralin concentration, treatment condition on in vitro culture of cabbage starting from seeds

Trait Effect MS F P
Rate of seed germination cultivar 11339.3 87.4 0.0000*
trifluralin concentration 33654.1 259.3 0.0000*
treatment condition 7613.4 58.668 0.0000*
treatment condition × cultivar 638.6 4.9213 0.0000*
treatment condition × concentration 382.4 2.9465 0.0015*
concentration × cultivar 925.4 7.131 0.000*
treatment × concentration × cultivar 237.4 1.829 0.00262*
Shoots per germinated seed cultivar 17.91 2.813 0.014*
trifluralin concentration 4.4 0.692 0.5990
treatment condition 6.5 1.021 0.3867
treatment condition × cultivar 10.05 1.5795 0.0799
treatment condition × concentration 9.34 1.4674 0.1492
concentration × cultivar 9.53 1.4972 0.0860
treatment × concentration × cultivar 6.96 1.094 0.337
Rate of root induction cultivar 10575.3 47.1 0.0000*
trifluralin concentration 23284.8 103.6 0.0000*
treatment condition 6557.2 29.186 0.0000*
treatment condition × cultivar 377.3 1.6793 0.05559
treatment condition × concentration 717.0 3.1912 0.00068*
concentration × cultivar 1636.5 7.2842 0.0000*
treatment × concentration × cultivar 446.7 1.988 0.000749*
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Inglese
Frequenza di pubblicazione:
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