In recent years, the use of bio-fertilizers enriched with specially selected microorganisms has been used more and more often. The beneficial effects of bio-fertilizers enriched with consortia of microorganisms on strawberry plants have been reported previously. The purpose of the research was to determine the effect of bio-fertilizers containing selected fungal and bacterial strains on the microorganisms living in the rhizosphere of strawberry plants. In the experiments described in this paper, synthetic mineral fertilizers were enriched with selected microorganisms. The fertilizer urea was enriched with the fungi