Accesso libero

Description of two new Pristionchus species from South Korea

Matthias Herrmann

Matthias Herrmann

Max Planck Institute for Biology Tübingen, Department of Integrative Evolutionary BiologyTübingen, Germany
Cerca questo autore su
Sciendo|Google Scholar
Herrmann, Matthias
, 
Natsumi Kanzaki

Natsumi Kanzaki

Kansai Research Center, Forestry and Forest Products Research Institute (FFPRI)Kyoto, Japan
Cerca questo autore su
Sciendo|Google Scholar
Kanzaki, Natsumi
, 
Christian Weiler

Christian Weiler

Max Planck Institute for Biology Tübingen, Department of Integrative Evolutionary BiologyTübingen, Germany
Cerca questo autore su
Sciendo|Google Scholar
Weiler, Christian
, 
Penghieng Theam

Penghieng Theam

Max Planck Institute for Biology Tübingen, Department of Integrative Evolutionary BiologyTübingen, Germany
Cerca questo autore su
Sciendo|Google Scholar
Theam, Penghieng
, 
Christian Rödelsperger

Christian Rödelsperger

Max Planck Institute for Biology Tübingen, Department of Integrative Evolutionary BiologyTübingen, Germany
Cerca questo autore su
Sciendo|Google Scholar
Rödelsperger, Christian
 e 
Ralf Sommer

Ralf Sommer

Max Planck Institute for Biology Tübingen, Department of Integrative Evolutionary BiologyTübingen, Germany
Cerca questo autore su
Sciendo|Google Scholar
Sommer, Ralf
  
04 ott 2024
Description of two new Pristionchus species from South Korea's Cover Image
INFORMAZIONI SU QUESTO ARTICOLO
Articolo precedente
Articolo Successivo
Cita
Scarica la copertina
Categoria dell'articolo: Research Paper
Pubblicato online: 04 ott 2024
Ricevuto: 03 lug 2024
DOI: https://doi.org/10.2478/jofnem-2024-0032
Parole chiave
© 2024 Matthias Herrmann et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1:

Molecular phylogeny of Pristionchus species. The phylogeny shows a maximum likelihood tree that was inferred from a concatenated alignments of more than 1200 orthologous gene clusters. Branch lengths reflect the estimated number of amino acid substitutions per site and branch labels show bootstrap support values (100 pseudoreplicates). The accession numbers next to the species names denote the Run accession in the European nucleotide Archive, where the raw RNA-seq reads were deposited.
Molecular phylogeny of Pristionchus species. The phylogeny shows a maximum likelihood tree that was inferred from a concatenated alignments of more than 1200 orthologous gene clusters. Branch lengths reflect the estimated number of amino acid substitutions per site and branch labels show bootstrap support values (100 pseudoreplicates). The accession numbers next to the species names denote the Run accession in the European nucleotide Archive, where the raw RNA-seq reads were deposited.

Figure 2:

Pristionchus hangukensis n. sp. A: Male; B: Female; C: Anterior region; D: Relative position of secretory-excretory pore, deirid and one of lateral glands; E: Surface of anterior end of adult male in right lateral view showing the relative position of labial sensilla, cephalic sensilla and amphid; E, F: Male stenostomatous form in left (E) and right (F) lateral views; G, H: Male eurystomatous form in left (G) and right (H) lateral views. Tooth and denticules are separately drawn under whole head region in E–H.
Pristionchus hangukensis n. sp. A: Male; B: Female; C: Anterior region; D: Relative position of secretory-excretory pore, deirid and one of lateral glands; E: Surface of anterior end of adult male in right lateral view showing the relative position of labial sensilla, cephalic sensilla and amphid; E, F: Male stenostomatous form in left (E) and right (F) lateral views; G, H: Male eurystomatous form in left (G) and right (H) lateral views. Tooth and denticules are separately drawn under whole head region in E–H.

Figure 3:

Pristionchus hangukensis n. sp. A, B: Whole male tail in right lateral (A) and ventral (B) views; C, D: Close-up of posterior four pairs of genital papillae and phasmid in right lateral (C) and ventral (D) views; E, F: Spicule and gubernaculum of two different individuals in right lateral view; G: Ventral view of female vulval region; H, I: Anal region of female in left lateral (H) and ventral (I) views.
Pristionchus hangukensis n. sp. A, B: Whole male tail in right lateral (A) and ventral (B) views; C, D: Close-up of posterior four pairs of genital papillae and phasmid in right lateral (C) and ventral (D) views; E, F: Spicule and gubernaculum of two different individuals in right lateral view; G: Ventral view of female vulval region; H, I: Anal region of female in left lateral (H) and ventral (I) views.

Figure 4:

Stomatal region of Pristionchus hangukensis n. sp. showing variation among individuals. A: Surface and stoma of male in left lateral view in five different focal planes showing the relative position of labial sensilla, cephalic sensilla, amphid, left subventral ridge and dorsal tooth; B, C: Male (B) and female (C) stenostomatous form in right lateral views in two different focal planes; D, E: Female eurystomatous form in left (D) and right (E) lateral views in two different focal planes. Abbreviations are as follows: am = amphid; cs = cephalic sensilla; d = dorsal tooth; ls = labial sensilla; lsv = left subventral ridge; rsv = right subventral ridge or tooth.
Stomatal region of Pristionchus hangukensis n. sp. showing variation among individuals. A: Surface and stoma of male in left lateral view in five different focal planes showing the relative position of labial sensilla, cephalic sensilla, amphid, left subventral ridge and dorsal tooth; B, C: Male (B) and female (C) stenostomatous form in right lateral views in two different focal planes; D, E: Female eurystomatous form in left (D) and right (E) lateral views in two different focal planes. Abbreviations are as follows: am = amphid; cs = cephalic sensilla; d = dorsal tooth; ls = labial sensilla; lsv = left subventral ridge; rsv = right subventral ridge or tooth.

Figure 5:

Male tail characters of Pristionchus hangukensis n. sp. A: Right lateral view of whole tail in three different focal planes; B: Left subventral view of whole tail; C: Spicule and gubernaculum in right lateral view in three focal planes. Abbreviations are as follows: ad, pd and v + number = genital papillae, where ending with ‘d’ suggests that the papillae are directed laterally or subdorsally; ph = phasmid; vs = ventral single papilla.
Male tail characters of Pristionchus hangukensis n. sp. A: Right lateral view of whole tail in three different focal planes; B: Left subventral view of whole tail; C: Spicule and gubernaculum in right lateral view in three focal planes. Abbreviations are as follows: ad, pd and v + number = genital papillae, where ending with ‘d’ suggests that the papillae are directed laterally or subdorsally; ph = phasmid; vs = ventral single papilla.

Figure 6:

Pristionchus coreanus n. sp. A: Male; B: Female; C: Anterior region; D: Relative position of secretory-excretory pore, deirid, and two lateral glands on the lateral field.
Pristionchus coreanus n. sp. A: Male; B: Female; C: Anterior region; D: Relative position of secretory-excretory pore, deirid, and two lateral glands on the lateral field.

Figure 7:

Stomatal region of Pristionchus coreanus n. sp. showing variation among individuals. A: Surface of anterior end of adult male in right lateral view showing the relative position of labial sensilla, cephalic sensilla and amphid; B, C: Male stenostomatous form starved culture in left (B) and right (C) lateral views; D, E: Female stenostomatous form from starved culture in left (D) and right (E) lateral views; F, G: Female stenostomatous form from well-fed culture in left (F) and right (G) lateral views. Tooth and denticules are separately drawn under whole head region in B–G.
Stomatal region of Pristionchus coreanus n. sp. showing variation among individuals. A: Surface of anterior end of adult male in right lateral view showing the relative position of labial sensilla, cephalic sensilla and amphid; B, C: Male stenostomatous form starved culture in left (B) and right (C) lateral views; D, E: Female stenostomatous form from starved culture in left (D) and right (E) lateral views; F, G: Female stenostomatous form from well-fed culture in left (F) and right (G) lateral views. Tooth and denticules are separately drawn under whole head region in B–G.

Figure 8:

Pristionchus coreanus n. sp. A, B: Whole male tail in right lateral (A) and ventral (B) views; C, D: Close-up of posterior four pairs of genital papillae and phasmid in right lateral (C) and ventral (D) views; E: Spicule and gubernaculum in right lateral view; F: Anterior gonad of mature female in right lateral view; G: Ventral view of female vulval region; H, I: Anal region of female in right lateral (H) and ventral (I) views.
Pristionchus coreanus n. sp. A, B: Whole male tail in right lateral (A) and ventral (B) views; C, D: Close-up of posterior four pairs of genital papillae and phasmid in right lateral (C) and ventral (D) views; E: Spicule and gubernaculum in right lateral view; F: Anterior gonad of mature female in right lateral view; G: Ventral view of female vulval region; H, I: Anal region of female in right lateral (H) and ventral (I) views.

Figure 9:

Stomatal region of stenostomatous form of Pristionchus coreanus n. sp. showing variation among individuals. A: Surface and stoma of male in left lateral view in three different focal planes showing the relative position of labial sensilla, cephalic sensilla and amphid; B: Left lateral view of male from well-fed culture in two different focal planes; C: Right lateral view of male from starved culture in two different focal planes; D: Left lateral view of female from starved culture in two different focal planes; E: Right lateral view of female from starved culture in two different focal planes; F: Left lateral view of female from well-fed culture in two different focal planes; G: Right lateral view of female from well-fed culture in two different focal planes. Abbreviations are as follows: am = amphid; cs = cephalic sensilla; d = dorsal tooth; ls = labial sensilla; lsv = left subventral ridge; rsv = right subventral ridge or tooth.
Stomatal region of stenostomatous form of Pristionchus coreanus n. sp. showing variation among individuals. A: Surface and stoma of male in left lateral view in three different focal planes showing the relative position of labial sensilla, cephalic sensilla and amphid; B: Left lateral view of male from well-fed culture in two different focal planes; C: Right lateral view of male from starved culture in two different focal planes; D: Left lateral view of female from starved culture in two different focal planes; E: Right lateral view of female from starved culture in two different focal planes; F: Left lateral view of female from well-fed culture in two different focal planes; G: Right lateral view of female from well-fed culture in two different focal planes. Abbreviations are as follows: am = amphid; cs = cephalic sensilla; d = dorsal tooth; ls = labial sensilla; lsv = left subventral ridge; rsv = right subventral ridge or tooth.

Figure 10:

Male tail characters of Pristionchus coreanus n. sp. Right lateral view of whole male tail in five different focal planes. Abbreviations are as follows: ad, pd and v + number = genital papillae where ending with ‘d’ suggests that the papillae are directed laterally or subdorsally; ph = phasmid; vs = ventral single papilla.
Male tail characters of Pristionchus coreanus n. sp. Right lateral view of whole male tail in five different focal planes. Abbreviations are as follows: ad, pd and v + number = genital papillae where ending with ‘d’ suggests that the papillae are directed laterally or subdorsally; ph = phasmid; vs = ventral single papilla.

Supplemental Figure 1:

18S tree for different Pristionchus species. The 18S rRNA sequences for the two new species were aligned together with their best hits from the NCBI database and a maximum likelihood tree was calculated using the phangorn package in R.
18S tree for different Pristionchus species. The 18S rRNA sequences for the two new species were aligned together with their best hits from the NCBI database and a maximum likelihood tree was calculated using the phangorn package in R.

Morphological measurements of the two new species_

Character Pristionchus coreanus n.sp. RS6268 Pristionchus hangukensis n.sp. RS6291
stenostomatous male stenostomatous female stenostomatous male stenostomatous female
n 10 10 10 10
L 1002 ± 94.3 (872 – 1158) 1448 ± 126.0 (1312 – 1661) 928 ± 54.8 (858 – 1001) 1336 ± 90.2 (1248 – 1542)
L’ 851 ± 93.3 (721 – 1025) 1211 ± 120.4 (1067 – 1442) 764 ± 41.0 (685 – 820) 1078 ± 76.5 (993 – 1249)
a 13 ± 2.2 (9.1 – 16) 12 ± 1.5 (11 – 16) 13 ± 1.3 (11 – 16) 13 ± 0.7 (11 – 14)
b 6.0 ± 0.4 (5.5 – 6.7) 7.6 ± 0.5 (6.8 – 8.4) 7.0 ± 0.4 (6.5 – 7.6) 9.0 ± 0.7 (8.0 – 10.0)
c 6.7 ± 0.9 (5.7 – 8.5) 6.1 ± 0.5 (5.4 – 7.1) 5.8 ± 0.4 (5.3 – 6.6) 5.3 ± 0.5 (4.9 – 6.3)
c’ 3.4 ± 0.5 (2.5 – 4.0) 4.4 ± 0.6 (3.8 – 5.5) 4.4 ± 0.5 (3.9 – 5.3) 6.4 ± 0.6 (5.5 – 7.7)
Ant. stoma length (cheilo- + gymnostom) 5.1 ± 0.8 (4.0 – 6.7) 5.7 ± 1.4 (4.0 – 7.8) 6.9 ± 1.1 (5.0 – 9.3) 7.7 ± 0.7 (6.7 – 9.1)
Total stoma length 11 ± 1.0 (9.3 – 12) 12 ± 1.1 (11 – 14) 10 ± 1.1 (8.3 – 11.6) 13 ± 1.0 (11 – 14)
Stoma width 5.8 ± 0.4 (5.3 – 6.5) 7.5 ± 0.9 (6.0 – 8.7) 4.9 ± 0.9 (3.6 – 6.5) 5.4 ± 0.9 (4.3 – 7.5)
Ant. pharynx (pro + metacorpus) 95 ± 7.6 (83 – 111) 109 ± 8.2 (99 – 130) 69 ± 5.6 (63 – 82) 79 ± 6.0 (72 – 89)
Post. pharynx (isthmus + basal bulb) 67 ± 7.0 (56 – 78) 77 ± 5.1 (69 – 87) 57 ± 2.9 (54 – 61) 61 ± 3.6 (55 – 66)
Total pharynx length 162 ± 13.6 (142 – 183) 186 ± 12.1 (176 – 217) 126 ± 7.6 (120 – 143) 140 ± 7.4 (131 – 152)
Ant./total pharynx % 59 ± 1.7 (57 – 62) 58 ± 1.5 (56 – 62) 55 ± 1.6 (52 – 57) 57 ± 2.2 (54 – 60)
Median bulb diameter 27 ± 2.5 (24 – 32) 35 ± 2.9 (31 – 40) 23 ± 1.2 (21 – 24) 27 ± 2.0 (25 – 31)
Terminal bulb diameter 28 ± 3.9 (22 – 36) 36 ± 4.9 (31 – 46) 22 ± 1.1 (20 – 23) 27 ± 2.3 (24 – 33)
Ant. end to cardia 167 ± 13.2 (151 – 191) 192 ± 8.8 (185 – 212) 132 ± 8.4 (123 – 148) 148 ± 7.5 (139 – 160)
Ant. end to S-E pore 149 ± 15.5 (125 – 170) 195 ± 15.5 (171 – 214) 114 ± 11.3 (99 – 139) 153 ± 12.0 (137 – 172)
Ant. end to nerve ring 118 ± 9.2 (109 – 133) 136 ± 8.9 (124 – 155) 97 ± 6.2 (90 – 108) 111 ± 6.5 (104 – 124)
Testis length 703 ± 83.8 (571 – 827) - 630 ± 59.4 (529 – 718) -
Ant. end to vulva distance - 691 ± 66.1 (600 – 821) - 608 ± 40.2 (563 – 703)
Vulva to anus distance - 525 ± 60.0 (454 – 628) - 473 ± 37.3 (420 – 543)
T or V 70 ± 4.3 (66 – 80) 48 ± 1.5 (46 – 50) 68 ± 6.7 (53 – 77) 46 ± 1.1 (43 – 47)
Max. body diameter 78 ± 19.1 (63 – 127) 121 ± 16.4 (91 – 141) 71 ± 5.1 (64 – 81) 105 ± 8.1 (93 – 115)
Cloacal or anal body diameter 45 ± 5.3 (40 – 54) 54 ± 5.3 (43 – 63) 36 ± 2.2 (33 – 39) 39 ± 3.5 (33 – 45)
Tail length 149 ± 10.8 (129 – 161) 237 ± 23.7 (208 – 296) 159 ± 13.9 (132 – 178) 252 ± 27.0 (204 – 297)
Spicule curve 50 ± 4.3 (40 – 54) - 46 ± 2.3 (43 – 50) -
Spicule chord 42 ± 2.9 (36 – 47) - 38 ± 2.6 (35 – 43) -
Gubernaculum length 18 ± 2.4 (13 – 21) - 18 ± 1.6 (16–22) -
Lingua:
Inglese
Frequenza di pubblicazione:
1 volte all'anno
Argomenti della rivista:
Scienze biologiche, Scienze della vita, altro
Feed RSS della rivista