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Exploring the use of metabarcoding to reveal eukaryotic associations with mononchids nematodes

Joseph O. Maosa

Joseph O. Maosa

Nematology Research Unit, Department of Biology, Ghent UniversityGhent, Belgium
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Maosa, Joseph O.
, 
Siqi Wang

Siqi Wang

Department of Plant Pathology, Nanjing Agricultural UniversityNanjing, China
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Wang, Siqi
, 
Shuhan Liu

Shuhan Liu

Department of Plant Pathology, Nanjing Agricultural UniversityNanjing, China
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Liu, Shuhan
, 
Hongmei Li

Hongmei Li

Department of Plant Pathology, Nanjing Agricultural UniversityNanjing, China
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Li, Hongmei
, 
Xue Qing

Xue Qing

Department of Plant Pathology, Nanjing Agricultural UniversityNanjing, China
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Qing, Xue
 e 
Wim Bert

Wim Bert

Nematology Research Unit, Department of Biology, Ghent UniversityGhent, Belgium
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Bert, Wim
  
10 mag 2024
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Categoria dell'articolo: Research Paper
Pubblicato online: 10 mag 2024
Ricevuto: 18 nov 2023
DOI: https://doi.org/10.2478/jofnem-2024-0016
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© 2024 Joseph O. Maosa et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1:

Detection of amplification fragments of rDNA 18S from 10 Mononchid species. A: Amplification with primer pair TAReuk454FWD1/TAReukREV3, lines 1–4 are M. brachyuris, C. parvus, Miconchus sp., and Prionchulus sp. respectively; B: Amplification with primer pair EUK20f/EUK302r+3, lines 1–6 are M. brevicaudatus, M. hawaiiensis, M. sigmaturellus, M. vulvapapillatus, Mylonchulus sp. and Anatonchus sp. respectively. M: DNA marker DL2000.
Detection of amplification fragments of rDNA 18S from 10 Mononchid species. A: Amplification with primer pair TAReuk454FWD1/TAReukREV3, lines 1–4 are M. brachyuris, C. parvus, Miconchus sp., and Prionchulus sp. respectively; B: Amplification with primer pair EUK20f/EUK302r+3, lines 1–6 are M. brevicaudatus, M. hawaiiensis, M. sigmaturellus, M. vulvapapillatus, Mylonchulus sp. and Anatonchus sp. respectively. M: DNA marker DL2000.

Figure 2:

Diversity of eukaryotic organisms presented in 10 mononchid species at phylum level. The classification system is based on the eukaryote classification system of Adl et al., (2012).
Diversity of eukaryotic organisms presented in 10 mononchid species at phylum level. The classification system is based on the eukaryote classification system of Adl et al., (2012).

Figure 3:

Diversity of eukaryotic organisms presented in 10 mononchid species at order level. The classification system is based on the eukaryote classification system of Adl et al., (2012).
Diversity of eukaryotic organisms presented in 10 mononchid species at order level. The classification system is based on the eukaryote classification system of Adl et al., (2012).

Figure S1:

Relative abundance of eukaryotic organisms presented in 10 mononchid species at different taxon levels. The percentages of each of the phyla or orders are calculated over the total number of reads excluding the nematode reads.
Relative abundance of eukaryotic organisms presented in 10 mononchid species at different taxon levels. The percentages of each of the phyla or orders are calculated over the total number of reads excluding the nematode reads.

Sampling information of Mononchid populations used in this study_

Species Habitat Sampling locality
M. brachyuris Grass land soil Linzhi, Tibetan
C. parvus Moss soil Zhouzhi, Shaanxi
Miconchus sp. Moss soil Nanjing, Jiangsu
Prionchulus sp. Populus sp. rhizosphere Qiqihar, Heilongjiang
M. brevicaudatus Moss soil Zhouzhi, Shaanxi
M. hawaiiensis Moss soil Nanjing, Jiangsu
M. sigmaturellus Digitaria sp. rhizosphere Nanyang, Henan
M. vulvapapillatus Moss soil Hulun Buir, Inner Mongolia
Mylonchulus sp. Populus sp. rhizosphere Qiqihar, Heilongjiang
Anatonchus sp. Moss soil Zhouzhi, Shaanxi

Primer pair used, generated reads, and ASVs per mononchid sample_

Species Reads ASVs Primer used
Kingdom Phylum Order
M. brachyuris 43 43 43 2 TAR
C. parvus 42 42 36 3 TAR
Miconchus sp. 2317 2315 2315 6 TAR
Prionchulus sp. 214 214 211 3 TAR
M. brevicaudatus 5983 5983 3618 7 EUK
M. hawaiiensis 43008 42672 40617 34 EUK
M. sigmaturellus 31737 30422 28781 89 EUK
M. vulvapapillatus 3944 3944 3929 13 EUK
Mylonchulus sp. 34869 34863 34864 15 EUK
Anatonchus sp. 35140 35140 34959 30 EUK
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