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First Morphological and Molecular Characterization of Paratylenchus vandenbrandei (Rhabditida: Tylenchulidae) in Iran

Abbas Abdolkhani
Abbas Abdolkhani
 e 
Sedighe Azimi
Sedighe Azimi
   | 14 dic 2023
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Article Category: Research Paper
Pubblicato online: 14 dic 2023
Pagine: -
Ricevuto: 04 ago 2023
DOI: https://doi.org/10.2478/jofnem-2023-0058
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© 2023 Abbas Abdolkhani et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1:

Line drawings of Paratylenchus vandenbrandei De Grisse, 1962. A, E, J: Male. A: Entire body; E: Anterior body region; J: Posterior body region; B-D, F-I: Female. B: Entire body; C, D: Anterior body region; F: Lateral field at mid-body; G-I: Posterior body region.
Line drawings of Paratylenchus vandenbrandei De Grisse, 1962. A, E, J: Male. A: Entire body; E: Anterior body region; J: Posterior body region; B-D, F-I: Female. B: Entire body; C, D: Anterior body region; F: Lateral field at mid-body; G-I: Posterior body region.

Figure 2:

Light photomicrographs of Paratylenchus vandenbrandei De Grisse, 1962. A, C-E, I-O: Female. A: Entire body; C-E: Anterior body region; I: Lateral field at mid-body; J, K: Reproductive system (the arrow indicates the spermatheca); L-O: Posterior body region (the arrow indicates the advulval flap); B, F-H, P, Q: Male. B: Entire body; F-H: Anterior body region; P, Q: Posterior body region. (Scale bars: A, B = 20 μm; C-Q = 10 μm).
Light photomicrographs of Paratylenchus vandenbrandei De Grisse, 1962. A, C-E, I-O: Female. A: Entire body; C-E: Anterior body region; I: Lateral field at mid-body; J, K: Reproductive system (the arrow indicates the spermatheca); L-O: Posterior body region (the arrow indicates the advulval flap); B, F-H, P, Q: Male. B: Entire body; F-H: Anterior body region; P, Q: Posterior body region. (Scale bars: A, B = 20 μm; C-Q = 10 μm).

Figure 3:

Light photomicrographs of Paratylenchus vandenbrandei De Grisse, 1962. Juvenile. A: Entire body; B, C: Anterior body region (the arrow indicates the excretory pore); D, E: Posterior body region. (Scale bars: A = 20 μm; B-E = 10 μm).
Light photomicrographs of Paratylenchus vandenbrandei De Grisse, 1962. Juvenile. A: Entire body; B, C: Anterior body region (the arrow indicates the excretory pore); D, E: Posterior body region. (Scale bars: A = 20 μm; B-E = 10 μm).

Figure 4:

Bayesian 50% majority rule consensus tree inferred from analysis of the D2-D3 domains of the LSU rDNA sequences of Paratylenchus vandenbrandei De Grisse, 1962. under the GTR + G + I model. Bayesian posterior probability values of more than 0.50 are given for appropriate clades. New sequences are indicated in bold.
Bayesian 50% majority rule consensus tree inferred from analysis of the D2-D3 domains of the LSU rDNA sequences of Paratylenchus vandenbrandei De Grisse, 1962. under the GTR + G + I model. Bayesian posterior probability values of more than 0.50 are given for appropriate clades. New sequences are indicated in bold.

Figure 5:

Bayesian 50% majority rule consensus tree inferred from analysis of the ITS rDNA of Paratylenchus vandenbrandei De Grisse, 1962. under the GTR + G + I model. Bayesian posterior probability values of more than 0.50 are given for appropriate clades. New sequences are indicated in bold.
Bayesian 50% majority rule consensus tree inferred from analysis of the ITS rDNA of Paratylenchus vandenbrandei De Grisse, 1962. under the GTR + G + I model. Bayesian posterior probability values of more than 0.50 are given for appropriate clades. New sequences are indicated in bold.

Morphometrics of Paratylenchus vandenbrandei from Khuzestan province, Iran, and comparison with the original description. All measurements are in μm and in the form: mean ± s.d. (range).

Character Present study De Grisse, 1962
Female Male Female Male
n 11 3 19 3
L 296 ±12 (259–314) 308 ± 53 (275–369) 269.0 ± 3.6 (248–290) 261 (248–269)
a 22.4 ± 0.8 (21.3–23.2) 29.6 ± 3.3 (27.2–32.0) 20 (14–24) 28 (27–30)
b 3.9 ± 0.2 (3.7–4.5) 4.4 ± 0.6 (4.2–4.6) 3.5 (3.2–3.7) -
c 20.0 ± 2.3 (15–22) 19.6 ± 1.9 (18.3–21.0) 18 (15–21) 16.6 (16.5–16.8)
c′ 2.0 ± 0.2 (1.6–2.3) 1.7 ± 0.07 (1.7–1.8) 2–3 -
V 80.0 ± 2.1 (79.0–84.5) - 82 (81–86) -
T - 19.2 ± 2.7 (17.3–21.2) - -
Stylet length 27 ± 1.7 (24.0–28.8) - 31.0 ± 5.6 (28–33) -
Conus length 17.1 ± 2.5 (14.7–19.5) - - -
m 71.1 ± 1.5 (65.1–74.2) - - -
DGO 3.5 ± 0.5 (3.2–4.2) - -
Anterior end to excretory pore 63.4 ± 3.0 (58–67) 61.0 ± 8.4 (55–67) 59–68 58–64
Pharynx length 74.6 ± 5.9 (68.5–81.2) 72.0 ± 9.8 (65–79) - -
Maximum body width 12.9 ± 0.8 (12.0–14.0) 10.9 ± 3.1 (10.1–12.5) - -
Vulva body width 12.2 ± 1.0 (11.5–13.5) - - -
Anal body width 7.5 ± 2.0 (6.5–9.2) 9.1 ± 0.8 (8.5–9.7) - -
Head-vulva 239.5 ± 8.5 (228–248) - - -
Head-anus 275.2 ±10.1 (264–292) - - -
Vulva-anus 39.5 ± 6.5 (29–48) - - -
St/L% 11.4 ± 0.7 (9.5–12.3) - - -
Tail length 15.1 ± 1.4 (14.0–17.5) 16.1 ± 1.5 (15.0–17.2) 15 (12–18) 15.6 (15–16)
Spicule length - 16.2 ± 1.7 (15.5–17.5) - 14.5 (14–15)
Gubernaculum length - 3.5 ± 1.7 (3.0–4.0) - 2.5–3.0
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