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Morphological and Molecular Characterization of Ottolenchus isfahanicus n. sp. (Tylenchomorpha: Tylenchoidea) from Rhizosphere of Gramineous Plants in Isfahan Province, Iran

Zeinab Mahboubi
Zeinab Mahboubi
, 
Mojtaba Keramat
Mojtaba Keramat
, 
Ebrahim Pourjam
Ebrahim Pourjam
, 
Mohammad Reza Atighi
Mohammad Reza Atighi
, 
Ilenia Clavero-Camacho
Ilenia Clavero-Camacho
, 
Joaquín Abolafia
Joaquín Abolafia
, 
Pablo Castillo
Pablo Castillo
 e 
Majid Pedram
Majid Pedram
   | 21 apr 2023
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Article Category: Research Paper
Pubblicato online: 21 apr 2023
Pagine: -
Ricevuto: 28 dic 2022
DOI: https://doi.org/10.2478/jofnem-2023-0011
Parole chiave
© 2023 Mahboubi et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1

Line drawings of Ottolenchus isfahanicus n. sp. (A, H: Male; B–G: Female). (A, B) Entire body. (C) Reproductive system (D) Anterior body region (E) Amphidial aperture. (F) Cross section at mid-body. (G, H) Tail and posterior body region.
Line drawings of Ottolenchus isfahanicus n. sp. (A, H: Male; B–G: Female). (A, B) Entire body. (C) Reproductive system (D) Anterior body region (E) Amphidial aperture. (F) Cross section at mid-body. (G, H) Tail and posterior body region.

Figure 3

Scanning electron microphotographs of Ottolenchus isfahanicus n. sp. (Female). (A–C, F) Lip region in ventral, lateral and face views, respectively (arrows pointing to the amphidial apertures). (D, E) Vulva in lateral and ventral views, respectively. (G) Excretory pore (arrow). (H) Anterior body region in ventral view (arrow pointing to the excretory pore). (I) Posterior body region (arrow pointing to the anus with liquid feces). (J) Tail (arrow pointing to the anus).
Scanning electron microphotographs of Ottolenchus isfahanicus n. sp. (Female). (A–C, F) Lip region in ventral, lateral and face views, respectively (arrows pointing to the amphidial apertures). (D, E) Vulva in lateral and ventral views, respectively. (G) Excretory pore (arrow). (H) Anterior body region in ventral view (arrow pointing to the excretory pore). (I) Posterior body region (arrow pointing to the anus with liquid feces). (J) Tail (arrow pointing to the anus).

Figure 2

Light microphotographs of Ottolenchus isfahanicus n. sp. (A–E, I: Female; F, G, H: Male). (A–C) Anterior body region showing pharynx, amphidial aperture and stylet, respectively. (D) Spermatheca. (E, H) Tail and tail tip (F, G) Spicule and bursa. (I) Lateral field at midbody. (All scale bars: B, C = 5 μm, rest = 10 μm).
Light microphotographs of Ottolenchus isfahanicus n. sp. (A–E, I: Female; F, G, H: Male). (A–C) Anterior body region showing pharynx, amphidial aperture and stylet, respectively. (D) Spermatheca. (E, H) Tail and tail tip (F, G) Spicule and bursa. (I) Lateral field at midbody. (All scale bars: B, C = 5 μm, rest = 10 μm).

Figure 4

Bayesian 50% majority rule consensus tree inferred from the SSU rDNA of Ottolenchus isfahanicus n. sp. under the GTR + G + I model (-ln L = 22872.6152; freq A = 0.1925; freqC = 0.2759; freqG = 0.3011; freq T = 0.2305; R(a) = 1.1309; R(b) = 2.9105; R(c) = 1.1816; R(d) = 0.9105; R(e) = 4.1153; R(f) = 1.0000; Pinva = 0.2877; Shape = 0.4556). Bayesian posterior probability values are given for corresponding clades. The new species is in bold font. GTR, general time-reversible; G, gamma; I, invariant; rDNA, ribosomal DNA; SSU, small subunit.
Bayesian 50% majority rule consensus tree inferred from the SSU rDNA of Ottolenchus isfahanicus n. sp. under the GTR + G + I model (-ln L = 22872.6152; freq A = 0.1925; freqC = 0.2759; freqG = 0.3011; freq T = 0.2305; R(a) = 1.1309; R(b) = 2.9105; R(c) = 1.1816; R(d) = 0.9105; R(e) = 4.1153; R(f) = 1.0000; Pinva = 0.2877; Shape = 0.4556). Bayesian posterior probability values are given for corresponding clades. The new species is in bold font. GTR, general time-reversible; G, gamma; I, invariant; rDNA, ribosomal DNA; SSU, small subunit.

Figure 5

Bayesian 50% majority rule consensus tree inferred from the LSU rDNA D2–D3 sequences of Ottolenchus isfahanicus n. sp. under the GTR + G + I model (-ln L = 24658.7734; freq A = 0.1395; freqC = 0.2523; freqG = 0.3373; freq T = 0.2709; R(a) = 1.1364; R(b) = 3.0722; R(c) = 1.3293; R(d) = 0.7313; R(e) = 3.9786; R(f) = 1.0000; Pinva = 0.1104; Shape = 0.6954). Bayesian posterior probability values are given for the corresponding clades. The new species is in bold font. GTR, general time-reversible; G, gamma; I, invariant; LSU, large subunit; rDNA, ribosomal DNA.
Bayesian 50% majority rule consensus tree inferred from the LSU rDNA D2–D3 sequences of Ottolenchus isfahanicus n. sp. under the GTR + G + I model (-ln L = 24658.7734; freq A = 0.1395; freqC = 0.2523; freqG = 0.3373; freq T = 0.2709; R(a) = 1.1364; R(b) = 3.0722; R(c) = 1.3293; R(d) = 0.7313; R(e) = 3.9786; R(f) = 1.0000; Pinva = 0.1104; Shape = 0.6954). Bayesian posterior probability values are given for the corresponding clades. The new species is in bold font. GTR, general time-reversible; G, gamma; I, invariant; LSU, large subunit; rDNA, ribosomal DNA.

Morphometrics of Ottolenchus isfahanicus n. sp. All measurements are in μm and in the form mean ± SD (range).

Characteristic Holotype
 Paratypes
Female Females Males
N 13 3
L 505 493.2 ± 12.1 (477-515) 430.3 ± 12.4 (416-438)
L’ 420 409.6 ± 10.3 (392-428) 353.3 ± 12.4 (339-361)
a 36.0 36.0 ± 2.2 (32.5-39.6) 37.8 ± 1.8 (35.7-39.1)
b 6.0 5.4 ± 0.2 (5.2-5.7) 4.9 ± 0.2 (4.7-5.0)
c 6.0 5.9 ± 0.4 (5.3-6.7) 5.6 ± 0.2 (5.4-5.6)
c’ 9.0 8.8 ± 0.7 (7.5-9.7) 9.7 ± 0.7 (8.9-10.2)
V or T 71.0 70.8 ± 1.0 (69.4-72.3) 80.0 ± 0.0 (81-82)
V’ 85.0 85.2 ± 1.0 (83.3-86.6) -
Lip region height 2 1.6 ± 0.2 (1.3-1.9) 1.7 ± 0.1 (1.5-1.7)
Lip region width 5 4.4 ± 0.3 (4.0-4.9) 4.5 ± 0.1 (4.4-4.6)
Stylet 6 6.3 ± 0.4 (5.7-6.9) 6.1 ± 0.3 (5.8-6.4)
Conus 2 1.8 ± 0.2 (1.5-2.2) 2.1 ± 0.2 (1.9-2.3)
Excretory pore 77 75.6 ± 2.2 (72-79) 68.3 ± 2.1 (66-70)
MB 38 37.5 ± 2.4 (32-40) 37.3 ± 2.5 (35-40)
Pharynx 91 91.3 ± 2.3 (88-95) 88.0 ± 5.3 (82-92)
Anterior end-vulva 358 394.2 ± 8.3 (337-361) 353.3 ± 12.4 (339-361)
Body width (BW) 14 13.7 ± 0.8 (13-15) 11.4 ± 0.2 (11.1-11.6)
Anal body width 10 9.5 ± 0.5 (9-10) 8 ± 0.6 (7.52-8.6)
Vulva-anus 62 60.5 ± 4.8 (55-68) 77 ± 0 (77-77)
Tail length 85 83.5 ± 6.0 (72-92) 11.1 ± 0.6 (10.5-11.5)
Tail/V-A 1 1.4 ± 0.2 (1.1-1.7) -
PUS 15 14.3 ± 1.2 (12.2-16.4) -
Spicules - - 11.1 ± 0.6 (10.5-11.5)
Gubernaculum - - 2.2 ± 0.9 (1.2-2.9)
Bursa length - - 20.3 ± 1.0 (19.3-21.2)
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Lingua:
Inglese
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