<abstract xmlns="http://www.w3.org/1999/xhtml">

<p><italic>Sauertylenchus maximus</italic> was discovered during a survey conducted at the Arlington National Cemetery, Virginia, for the type specimens of <italic>Hoplolaimus galeatus</italic>. Besides the fresh material, the fixed specimens of <italic>S. maximus</italic> were also studied by molecular and morphological means. The morphological and morphometric characteristics of the recovered fresh material were consistent with the original and other description(s) of this species. The fixed specimens used in this study were preserved in a 3% formaldehyde and 2% glycerin solution for over 20 yr. Molecular analyses of the fresh and fixed specimens were performed using internal transcribed spacer, D2–D2 expansion segments of 28S large subunits, and 18S small subunit ribosomal DNA sequences. To our knowledge, this represents the first report of <italic>S. maximus</italic> from Virginia and the first report of a successful DNA extraction from fixed nematode specimens.</p></abstract>

Sauertylenchus maximus was discovered during a survey conducted at the Arlington National Cemetery, Virginia, for the type specimens of Hoplolaimus galeatus. Besides the fresh material, the fixed specimens of S. maximus were also studied by molecular and morphological means. The morphological and morphometric characteristics of the recovered fresh material were consistent with the original and other description(s) of this species. The fixed specimens used in this study were preserved in a 3% formaldehyde and 2% glycerin solution for over 20 yr. Molecular analyses of the fresh and fixed specimens were performed using internal transcribed spacer, D2–D2 expansion segments of 28S large subunits, and 18S small subunit ribosomal DNA sequences. To our knowledge, this represents the first report of S. maximus from Virginia and the first report of a successful DNA extraction from fixed nematode specimens.

Morphological Characterization of Fresh and 20-Yr-Old Fixed Nematode Specimens of <italic>Sauertylenchus maximus</italic> (Allen, 1955) <xref ref-type="bibr" rid="j_jofnem-2022-0041_ref_026">Siddiqi, 2000</xref> Deposited in the USDA Nematode Collection from Arlington National Cemetery, VA, USA

Journal of Nematology

This work is licensed under the Creative Commons Attribution 4.0 International License.

Morphological Characterization of Fresh and 20-Yr-Old Fixed Nematode Specimens of  (Allen, 1955)  Deposited in the USDA Nematode Collection from Arlington National Cemetery, VA, USA

Sauertylenchus maximus was discovered during a survey conducted at the Arlington National Cemetery, Virginia, for the type specimens of Hoplolaimus galeatus....

Morphological Characterization of Fresh and 20-Yr-Old Fixed Nematode Specimens of Sauertylenchus maximus (Allen, 1955) Siddiqi, 2000 Deposited in the USDA Nematode Collection from Arlington National Cemetery, VA, USA

Article Category: Research Paper

Pubblicato online: 19 ott 2022

Pagine: -

Ricevuto: 26 apr 2022

DOI: https://doi.org/10.2478/jofnem-2022-0041

Parole chiave
18S rDNA, 28S rDNA, fixed specimens, ITS rDNA, taxonomy, USDANC

© 2022 Kantor et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Morphological Characterization of Fresh and 20-Yr-Old Fixed Nematode Specimens of Sauertylenchus maximus (Allen, 1955) Siddiqi, 2000 Deposited in the USDA Nematode Collection from Arlington National Cemetery, VA, USA

Article Category: Research Paper

Pubblicato online: 19 ott 2022

Pagine: -

Ricevuto: 26 apr 2022

DOI: https://doi.org/10.2478/jofnem-2022-0041

Parole chiave18S rDNA, 28S rDNA, fixed specimens, ITS rDNA, taxonomy, USDANC

© 2022 Kantor et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Parole chiave
18S rDNA, 28S rDNA, fixed specimens, ITS rDNA, taxonomy, USDANC