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The objective of the work is to evaluate the effects of a granulated feeding mixture enriched with knotweed (Reynoutria × bohemica) on roe deer (Capreolus capreolus) bred at a closed farm and red deer (Cervus elaphus) bred at a farm. Based on both biochemical and haematological blood analysis, the knotweed is expected to have an influence on the microbiome in the digestive system as well as allowing better utilisation of fodder and lower manifestation of pathogenic organisms. The results are of practical use mainly on farms and in hobby breeding but also in deer parks. Furthermore, the results may be used for feeding deer in open hunting grounds.