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Fig. 1
Gram staining of Melissococcus plutonius strain ATCC 35311, Cultivated under anaerobic conditions in SBK medium for 5 days at 36°C.
Fig. 2
Melissococcus plutonius infection cycle 1. egg, 2. Ingestion of M. plutonius (Mp) in the royal jelly (RJ). 3. Proliferation of M. plutonius in the intestine, 4a. Manifestation of EFB symptoms, 4b Larvae without EFB symptoms, 5a. Death of larvae, scale formation and brood removal by worker bees, 5b. Sealing cell and slow pupae development, 5c. Death of larva after cell was seal, 6a. Cell with M. plutonius and secondary agents, 6b. Emergence of smaller adults carrying M. plutonius and cells with M. plutonius and secondary agents. Adapted from Bailey & Ball (1991).