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Histochemical Staining of Acetylcholinesterase in Carnolian Honeybee (Apis mellifera carnica) Brain after Chronic Exposure to Organophosphate Diazinon

Gordana Glavan

Gordana Glavan

University of Ljubljana, Biotechnical faculty, Department of BiologyLjubljana, Slovenia
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Glavan, Gordana
  
02 lug 2020
Histochemical Staining of Acetylcholinesterase in Carnolian Honeybee (Apis mellifera carnica) Brain after Chronic Exposure to Organophosphate Diazinon's Cover Image
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Categoria dell'articolo: Original Article
Pubblicato online: 02 lug 2020
Pagine: 123 - 130
Ricevuto: 09 ott 2019
Accettato: 07 gen 2020
DOI: https://doi.org/10.2478/jas-2020-0003
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© 2020 Gordana Glavan, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

AChE histochemistry in honeybee antennal lobes (A, B), mushroom body (C, D) and optic lobes (E, F) after diazinon treatment with compared to the control. al, antennal lobe; glo, glomeruli of antennal lobus; an; antennal nerve; cal, calyces; lip, lip of calyces; la, lamina; me, medulla; lo, lobula; s, somata; och, outer chiazma; ich, inner chiazma; c, c-layer; α, α-lobe of mushroom body; β, β-lobe of mushroom body; lateral protocerebrum (lp). Scales 100 µm (antennal lobus: A, B, and mushroom body: C, D) and 20µm (optic lobes: E, F).
AChE histochemistry in honeybee antennal lobes (A, B), mushroom body (C, D) and optic lobes (E, F) after diazinon treatment with compared to the control. al, antennal lobe; glo, glomeruli of antennal lobus; an; antennal nerve; cal, calyces; lip, lip of calyces; la, lamina; me, medulla; lo, lobula; s, somata; och, outer chiazma; ich, inner chiazma; c, c-layer; α, α-lobe of mushroom body; β, β-lobe of mushroom body; lateral protocerebrum (lp). Scales 100 µm (antennal lobus: A, B, and mushroom body: C, D) and 20µm (optic lobes: E, F).

Fig. 2

The effects of diazinon on AChE activity in different regions of honeybee brain. AL, antennal lobe; MB-lip, lip of calyces in mushroom body, OL-LA, lamina of optic lobes; OL-ME, medulla of optic lobes; OL-LO, lobula of optic lobes; LP-lateral protocerebrum. Data are presented as optical density (OD); mean ± standard deviation (SD). Number of bees: N=13 for control group and N=8 for diazinon-treated group;*significantly different from the control (unpaired t-test, p<0.05).
The effects of diazinon on AChE activity in different regions of honeybee brain. AL, antennal lobe; MB-lip, lip of calyces in mushroom body, OL-LA, lamina of optic lobes; OL-ME, medulla of optic lobes; OL-LO, lobula of optic lobes; LP-lateral protocerebrum. Data are presented as optical density (OD); mean ± standard deviation (SD). Number of bees: N=13 for control group and N=8 for diazinon-treated group;*significantly different from the control (unpaired t-test, p<0.05).
Lingua:
Inglese
Frequenza di pubblicazione:
2 volte all'anno
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Scienze biologiche, Zoologia, Scienze della vita, altro
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