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Helminthologia
Volume 57 (2020): Numero 2 (June 2020)
Accesso libero
Molecular characterization and phylogenetic analysis of
Toxocara
species in dogs, cattle and buffalo in Egypt
O.A. Mahdy
O.A. Mahdy
,
W. M. Mousa
W. M. Mousa
,
S. Z. Abdel-Maogood
S. Z. Abdel-Maogood
,
S. M. Nader
S. M. Nader
e
S. Abdel-Radi
S. Abdel-Radi
| 23 mag 2020
Helminthologia
Volume 57 (2020): Numero 2 (June 2020)
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CONDIVIDI
Pubblicato online:
23 mag 2020
Pagine:
83 - 90
Ricevuto:
20 mag 2019
Accettato:
21 dic 2019
DOI:
https://doi.org/10.2478/helm-2020-0013
Parole chiave
molecular characterization
,
PCR-mitochondrial gene- ribosomal gene
,
dogs
,
cattle
,
buffalo
,
Egypt
© 2020 O. A. Mahdy, W. M. Mousa, S. Z. Abdel-Maogood, S. M. Nader, S. Abdel-Radi, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.
Fig. 1
A – B. Toxocara vitulorum collected from calves. B) Anterior end of worm showed lips (short white arrow), posterior end of male showed coiled tail (black arrow), and posterior end of female worm showed a straight tail end (long white arrow). D) Anterior end of T.vitulorum showed three lips (lip), F) posterior end of female showed short tail (st), G) posterior end of male showed two spicules (sp), C) Toxocara canis collected from street dogs; showed the coiled tail-end of male (black arrow), the straight tail-end of female (white arrow), E) Anterior end of T. canis showed the cephalic alae and lips, F) posterior end of male showed spicules.
Plate 1
Results of agarose gel electrophoresis of the PCR products of the selected genes in Toxocara spp. showing the M. W. of each gene band.
Plate 2
Phylogenetic tree constructed in MEGA by neighbor- joining method for the ATPase-6 region of the tested Toxocara spp.
Plate 3
Phylogenetic tree constructed in MEGA by neighbor-joining method for the 12S region of the tested Toxocara spp.
Plate 4
Phylogenetic tree constructed in MEGA by neighbor- joining method for the ITS-2 region of the tested Toxocara spp.
Characterization of the PCR products of the selected genes in the tested Toxocara spp. showing the M. W. of the bands for each gene.
Gene
M.W. (bp)
T. canis
T. vitulorum
from cattle
T. vitulorum
from buffalo
ATPase-6
804
828
828
12S
572
576
572
ITS-2
592
625
625