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Impact of g-Load Shift on Temporal Expression Pattern of Apoptosis-linked Proteins in the Rat Mammary Gland

Kibrom M. Alula

Kibrom M. Alula

Department of Cell and Molecular Biology, Grand Valley State UniversityAllendale, United States
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Alula, Kibrom M.
, 
James H. Resau

James H. Resau

BioSpecimen Science, Van Andel InstituteGrand Rapids, United States
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Resau, James H.
 e 
Osman V. Patel

Osman V. Patel

Department of Cell and Molecular Biology, Grand Valley State UniversityAllendale, United States
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Patel, Osman V.
  
17 giu 2019
Impact of g-Load Shift on Temporal Expression Pattern of Apoptosis-linked Proteins in the Rat Mammary Gland's Cover Image
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Categoria dell'articolo: Short Communication
Pubblicato online: 17 giu 2019
Pagine: 13 - 21
DOI: https://doi.org/10.2478/gsr-2019-0003
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© 2019 Kibrom M. Alula, James H. Resau, Osman V. Patel, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Figure 1

Representative images of lobular outlining (green) and unmixing for protein quantification. An overlay image (a) was unmixed into blue hematoxylin (b) and red VDR (c). The unmixed images of dual-labeled slide enable quantification of individual, as well as overlapping signals within the same outlined lobular border. The scale bar represents 50 mm (10× magnification) in the photomicrographs. VDR, vitamin D receptor.
Representative images of lobular outlining (green) and unmixing for protein quantification. An overlay image (a) was unmixed into blue hematoxylin (b) and red VDR (c). The unmixed images of dual-labeled slide enable quantification of individual, as well as overlapping signals within the same outlined lobular border. The scale bar represents 50 mm (10× magnification) in the photomicrographs. VDR, vitamin D receptor.

Figure 2

Quantitative comparison of mammary gland lobular CC-3 (a: within groups; b: between groups), P53 (c: within groups; d: between groups), and VDR (e: within groups; f: between groups) proteins measured in SC and HG-exposed rats following unmixing of chromophores using the CRi Nuance Multispectral Imaging System at G20, P1, and P3. Data are shown as mean ± SEM; *significantly different within and between each group (*p<0.05; **p<0.005; ***p<0.001; ****p<0.0001).G20, day 20 of gestation; HG, hypergravity; P1, postpartum day 1; P3, postpartum day 3; P53, tumor protein p53; SC, stationary control; SEM, standard error of the mean; VDR, vitamin D receptor.
Quantitative comparison of mammary gland lobular CC-3 (a: within groups; b: between groups), P53 (c: within groups; d: between groups), and VDR (e: within groups; f: between groups) proteins measured in SC and HG-exposed rats following unmixing of chromophores using the CRi Nuance Multispectral Imaging System at G20, P1, and P3. Data are shown as mean ± SEM; *significantly different within and between each group (*p<0.05; **p<0.005; ***p<0.001; ****p<0.0001).G20, day 20 of gestation; HG, hypergravity; P1, postpartum day 1; P3, postpartum day 3; P53, tumor protein p53; SC, stationary control; SEM, standard error of the mean; VDR, vitamin D receptor.
Lingua:
Inglese
Frequenza di pubblicazione:
2 volte all'anno
Argomenti della rivista:
Scienze biologiche, Scienze della vita, altro, Scienze materiali, Scienze materiali, altro, Fisica, Fisica, altro
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