Corporate headquarters, which occupy the top of enterprise organizational hierarchies became the subject of an increased interest in last three decades. Until now, posttransformation economies suffer from a distinct cognitive gap in this respect. The main objective of this paper is to analyse and interpret the development of the spatial pattern of top 100 biggest companies in the Czech Republic. Both quantitative and qualitative dimension of this issue will be investigated in the frame of this article. The basic territorial level we examine is constituted by self-governing regions. Finally, particular managerial and regionally-orientated recommendations for both largest enterprises and public administration will be formulated.