<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>The elements are present in the environment. Moreover, they are used in pharmacy and the production of new materials used in medical applications. They are often as environmental pollutants. They can accumulate in organisms and induce toxic effects on the cellular level. HepG2, L<sub>929</sub> and Caco-2 cell lines were exposed to known concentrations of chromium chloride, iron chloride, nickel chloride, molybdenum trioxide and cobalt chloride (200 or 1000 μ M used alone and in combinations). Concentrations of chromium, iron, nickel, molybdenum and cobalt in the cell lysate and the culture medium were determined by ICP-MS. Moreover, sodium, potassium, calcium and magnesium concentrations were also measured. What is more, cells were observed under light and scanning electron microscope. The dose-dependent increase in the concentration of chromium, iron, nickel, molybdenum and cobalt in all cell lines after incubation with elements was observed. Potassium concentration decreases while sodium calcium and magnesium increase after incubation of cells with of mentioned elements. The incubation of cells with microelements induces cell morphology changes. The presented study shows the crucial role of tested microelements in the induction of cell death as a result of an imbalance of sodium, potassium, calcium and magnesium concentration inside the cell.</p>
</abstract>

The elements are present in the environment. Moreover, they are used in pharmacy and the production of new materials used in medical applications. They are often as environmental pollutants. They can accumulate in organisms and induce toxic effects on the cellular level. HepG2, L929 and Caco-2 cell lines were exposed to known concentrations of chromium chloride, iron chloride, nickel chloride, molybdenum trioxide and cobalt chloride (200 or 1000 μ M used alone and in combinations). Concentrations of chromium, iron, nickel, molybdenum and cobalt in the cell lysate and the culture medium were determined by ICP-MS. Moreover, sodium, potassium, calcium and magnesium concentrations were also measured. What is more, cells were observed under light and scanning electron microscope. The dose-dependent increase in the concentration of chromium, iron, nickel, molybdenum and cobalt in all cell lines after incubation with elements was observed. Potassium concentration decreases while sodium calcium and magnesium increase after incubation of cells with of mentioned elements. The incubation of cells with microelements induces cell morphology changes. The presented study shows the crucial role of tested microelements in the induction of cell death as a result of an imbalance of sodium, potassium, calcium and magnesium concentration inside the cell.

The Influence of Selected Transition Elements on Cell Metabolism

Ecological Chemistry and Engineering S

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

{"article-title":"The Influence of Selected Transition Elements on Cell Metabolism"}

The elements are present in the environment. Moreover, they are used in pharmacy and the production of new materials used in medical applications. They are...

The Influence of Selected Transition Elements on Cell Metabolism

Pubblicato online: 16 gen 2024

Pagine: 471 - 488

DOI: https://doi.org/10.2478/eces-2023-0043

Parole chiave
chromium, iron, nickel, molybdenum, cobalt, cell metabolism, ICP-MS, SEM, biomaterials

© 2023 Sylwia Terpiłowska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

The Influence of Selected Transition Elements on Cell Metabolism

Pubblicato online: 16 gen 2024

Pagine: 471 - 488

DOI: https://doi.org/10.2478/eces-2023-0043

Parole chiavechromium, iron, nickel, molybdenum, cobalt, cell metabolism, ICP-MS, SEM, biomaterials

© 2023 Sylwia Terpiłowska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Parole chiave
chromium, iron, nickel, molybdenum, cobalt, cell metabolism, ICP-MS, SEM, biomaterials