<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Facilities and equipment are described for large-scale, long-term “nose-only” inhalation exposure of mice to whole cigarette smoke. Experimental procedures and equipment were designed to provide the mice with exposure conditions where [1] the lung was the major target organ for the smoke, [2] large quantities of fresh, whole cigarette smoke could be generated, [3] large numbers of animals could be exposed at one time, [4] routine, daily exposures could be given over a major portion of the lifetime of the animal, [5] monitoring and documentation of the quantity of smoke presented to the animals was provided during each exposure session, [6] safety systems were provided that assured exposure of the animals to smoke only under pre-set exposure conditions, and [7] cigarette smoke was generated under conditions where factors, such as cigarette type, smoke aerosol concentration and smoke particle size, were controlled.</p></abstract>

Facilities and equipment are described for large-scale, long-term “nose-only” inhalation exposure of mice to whole cigarette smoke. Experimental procedures and equipment were designed to provide the mice with exposure conditions where [1] the lung was the major target organ for the smoke, [2] large quantities of fresh, whole cigarette smoke could be generated, [3] large numbers of animals could be exposed at one time, [4] routine, daily exposures could be given over a major portion of the lifetime of the animal, [5] monitoring and documentation of the quantity of smoke presented to the animals was provided during each exposure session, [6] safety systems were provided that assured exposure of the animals to smoke only under pre-set exposure conditions, and [7] cigarette smoke was generated under conditions where factors, such as cigarette type, smoke aerosol concentration and smoke particle size, were controlled.

Chronic Inhalation Studies in Mice: I. Facilities and Equipment for “Nose-Only” Exposure to Cigarette Smoke

Division of Toxicology and Molecular Biology, Microbiological Associates, Bethesda, Maryland, U.S.A.,

Analytical Chemistry Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, U.S.A.,

Process and Instruments Corporation, Brooklyn, New York, U.S.A.

Contributions to Tobacco & Nicotine Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Facilities and equipment are described for large-scale, long-term “nose-only” inhalation exposure of mice to whole cigarette smoke. Experimental procedures and...

Chronic Inhalation Studies in Mice: I. Facilities and Equipment for “Nose-Only” Exposure to Cigarette Smoke

Pubblicato online: 06 gen 2015

Pagine: 37 - 53

Ricevuto: 30 apr 1984

Accettato: 15 ott 1984

DOI: https://doi.org/10.2478/cttr-2013-0554

© 1985 Beiträge zur Tabakforschung International

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.