<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Brain-derived neurotrophic factor (BDNF) has an important role in energy balance. It suppresses food intake, reduces hepatic glucose production and converts white fat into brown fat in adipose tissue, leading to energy dissipation, lowered blood glucose and a lean phenotype. Studies have shown that the single nucleotide polymorphism (SNP) Val66Met within BDNF may be associated with obesity, insulin sensitivity, type 2 diabetes mellitus (T2DM) and dyslipidemia. The objective of the study was to investigate the association of the Val66Met polymorphism with body mass index (BMI), fasting glucose levels and lipid profile in Serbian adolescents. The study included 308 randomly selected healthy adolescents, 153 (49.68%) boys and 155 girls (50.32%), 15 years of age. Data including age, gender, height, weight, lipid profile and fasting glucose were recorded. Genotyping was performed by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. No association of this polymorphism was found with BMI and lipid profile. However, significant association was observed between this polymorphism and fasting blood glucose (FBG). Carriers of a Val/Val genotype had significantly higher mean values of fasting glucose level compared to carriers of Val/ Met and Met/Met genotypes (<italic>p</italic> = 0.01). To confirm these results multiple linear regression analysis was performed. Body mass index and gender were taken as covariates. Carriers of the Val/Val genotype had significantly higher levels of FBG (β <italic>= -</italic>0.152<italic>, p</italic> = 0.02). A statistically significant association between BMI and glucose level was also observed (β = 0.124,<italic>p</italic> = 0.033). This polymorphism could be associated with fasting glucose level in Serbian adolescents, thus further research would be of great interest to validate these results.</p></abstract>

Brain-derived neurotrophic factor (BDNF) has an important role in energy balance. It suppresses food intake, reduces hepatic glucose production and converts white fat into brown fat in adipose tissue, leading to energy dissipation, lowered blood glucose and a lean phenotype. Studies have shown that the single nucleotide polymorphism (SNP) Val66Met within BDNF may be associated with obesity, insulin sensitivity, type 2 diabetes mellitus (T2DM) and dyslipidemia. The objective of the study was to investigate the association of the Val66Met polymorphism with body mass index (BMI), fasting glucose levels and lipid profile in Serbian adolescents. The study included 308 randomly selected healthy adolescents, 153 (49.68%) boys and 155 girls (50.32%), 15 years of age. Data including age, gender, height, weight, lipid profile and fasting glucose were recorded. Genotyping was performed by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. No association of this polymorphism was found with BMI and lipid profile. However, significant association was observed between this polymorphism and fasting blood glucose (FBG). Carriers of a Val/Val genotype had significantly higher mean values of fasting glucose level compared to carriers of Val/ Met and Met/Met genotypes (p = 0.01). To confirm these results multiple linear regression analysis was performed. Body mass index and gender were taken as covariates. Carriers of the Val/Val genotype had significantly higher levels of FBG (β = -0.152, p = 0.02). A statistically significant association between BMI and glucose level was also observed (β = 0.124,p = 0.033). This polymorphism could be associated with fasting glucose level in Serbian adolescents, thus further research would be of great interest to validate these results.

Association of the brain-derived neurotrophic factor Val66Met polymorphism with body mass index, fasting glucose levels and lipid status in adolescents

Balkan Journal of Medical Genetics

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Brain-derived neurotrophic factor (BDNF) has an important role in energy balance. It suppresses food intake, reduces hepatic glucose production and converts...

Parameters	Total (n = 308) (range)	Boys (n = 153)	Girls (n = 155)	p Value
BMI (kg/m²)	22.03±4.25 (13.8-43.72)	21.77±4.22	22.29±4.29	0.405
FBG (mmol/L)	4.68±0.63 (2.43-6.30)	4.75±0.59	4.61±0.66	0.660
TG (mmol/L)	0.98±0.55 (0.32-2.54)	0.97±0.62	0.99±0.47	0.891
TC (mmol/L)	4.35±0.86 (2.64-6.70)	4.18±0.76	4.51±0.93	0.001
HDL-C (mmol/L)	1.41±0.40 (0.28-2.63)	1.37±0.42	1.45±0.38	0.159
LDL-C (mmol/L)	2.47±0.86 (0.53-4.82)	2.39±0.81	2.55±0.90	0.057

Parameters	Genotype	Total	p Value	Boys	p Value	Girls	p Value
BMI (kg/m²)	GG GA+AA	22.17±4.37 21.76±3.82	0.378	21.79±4.14 21.74±4.40	0.715	22.54±4.57 21.80±3.67	0.367
FBG (mmol/L)	GG GA+AA	4.74±0.66 4.56±0.56	0.010	4.79±0.62 4.68±0.55	0.302	4.70±0.70 4.43±0.55	0.023
TG (mmol/L)	GG GA+AA	0.99±0.52 0.97±0.59	0.599	0.96±0.54 0.99±0.75	0.679	1.02±0.52 0.95±0.36	0.624
TC (mmol/L)	GG GA+AA	4.36±0.86 4.34±0.88	0.931	4.18±0.74 4.20±0.82	0.848	4.53±0.93 4.48±0.94	0.756
HDL-C (mmol/L)	GG GA+AA	1.42±0.42 1.44±0.44	0.484	1.34±0.34 1.42±0.53	0.580	1.45±0.41 1.48±0.33	0.659
LDL-C (mmol/L)	GG GA+AA	2.47±0.87 2.48±0.84	0.911	2.38±0.85 2.40±0.80	0.879	2.55±0.93 2.57±0.84	0.873

BDNF rs6265 Genotypes	Frequency n (%)	Alleles	Frequencies (%)
GG	202 (65.6)	G	0.82
GA	103 (33.4)	–	–
AA	3 (1-0)	A	0.18

Association of the brain-derived neurotrophic factor Val66Met polymorphism with body mass index, fasting glucose levels and lipid status in adolescents

Article Category: Original Article

Pubblicato online: 26 ago 2020

Pagine: 77 - 82

DOI: https://doi.org/10.2478/bjmg-2020-0004

Parole chiave
Body mass index (BMI), Brain-derived neurotrophic factor (BDNF), Fasting glucose level, Gene polymorphism, Lipid profile

© 2020 Vidović V, Maksimović N, Novaković I, Damnjanović T, Jekić B, Vidović S, Majkić Singh N, Stamenković-Radak M, Nikolić D, Marisavljević D, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mean values and range of analyzed parameters depending on gender.

Mean values of analyzed parameters by BDNF rs6265 genotype (mean±SD).

Frequencies of BDNF genotypes and alleles.

Association of the brain-derived neurotrophic factor Val66Met polymorphism with body mass index, fasting glucose levels and lipid status in adolescents

Article Category: Original Article

Pubblicato online: 26 ago 2020

Pagine: 77 - 82

DOI: https://doi.org/10.2478/bjmg-2020-0004

Parole chiaveBody mass index (BMI), Brain-derived neurotrophic factor (BDNF), Fasting glucose level, Gene polymorphism, Lipid profile

© 2020 Vidović V, Maksimović N, Novaković I, Damnjanović T, Jekić B, Vidović S, Majkić Singh N, Stamenković-Radak M, Nikolić D, Marisavljević D, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mean values and range of analyzed parameters depending on gender.

Mean values of analyzed parameters by BDNF rs6265 genotype (mean±SD).

Frequencies of BDNF genotypes and alleles.

Parole chiave
Body mass index (BMI), Brain-derived neurotrophic factor (BDNF), Fasting glucose level, Gene polymorphism, Lipid profile