<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Meningioma is the second most common adult central nervous system tumor. Mutations and/or deletions within the tumor suppressor gene neurofibromatosis type 2 (NF2) are associated with meningioma development and progression. We studied 29 meningioma samples by cytogenetic analysis and interphase fluorescence in situ hybridization (I-FISH) using a locus-specific probe for the NF2 gene region. We detected loss of the NF2 gene in all samples except for one. In 10 of the 29 samples, karyotypic analyses confirmed the I-FISH results and revealed additional numerical and/or structural rearrangements in nine of them. Our study confirmed: i) the limited role of banding cytogenetics in assessing chromosomal rearrangements in meningioma, as this tumor is hard to be grown in cell culture; ii) we could show that two-color I-FISH is well-suited for NF2-deletion screening. Our results were in accordance with those of comparable studies, even though the frequency of 97.0% of meningiomas with NF2 deletions is exceptionally high in the studied Sudanese patients</p></abstract>

Meningioma is the second most common adult central nervous system tumor. Mutations and/or deletions within the tumor suppressor gene neurofibromatosis type 2 (NF2) are associated with meningioma development and progression. We studied 29 meningioma samples by cytogenetic analysis and interphase fluorescence in situ hybridization (I-FISH) using a locus-specific probe for the NF2 gene region. We detected loss of the NF2 gene in all samples except for one. In 10 of the 29 samples, karyotypic analyses confirmed the I-FISH results and revealed additional numerical and/or structural rearrangements in nine of them. Our study confirmed: i) the limited role of banding cytogenetics in assessing chromosomal rearrangements in meningioma, as this tumor is hard to be grown in cell culture; ii) we could show that two-color I-FISH is well-suited for NF2-deletion screening. Our results were in accordance with those of comparable studies, even though the frequency of 97.0% of meningiomas with NF2 deletions is exceptionally high in the studied Sudanese patients

MOLECULAR CYTOGENETIC STUDY OF THE NF2 GENE DELETION IN MENINGIOMA IN SUDANESE PATIENTS

Zoology Department, Faculty of Science, Khartoum University, Khartoum, Sudan

Faculty of Medicine, Neilein University, Khartoum, Sudan

Jena University Hospital, Friedrich Schiller University, Institute of Human Genetics, Jena, Germany

Balkan Journal of Medical Genetics

Meningioma is the second most common adult central nervous system tumor. Mutations and/or deletions within the tumor suppressor gene neurofibromatosis type 2...

MOLECULAR CYTOGENETIC STUDY OF THE NF2 GENE DELETION IN MENINGIOMA IN SUDANESE PATIENTS

Pubblicato online: 11 mar 2014

Pagine: 29 - 32

DOI: https://doi.org/10.2478/bjmg-2013-0028

Parole chiave
Meningioma, NF2 Gene, Tumor suppressor gene, Banding cytogenetics, Interphase fluorescence in situ hybridization (I-FISH), Sudan

This content is open access.

MOLECULAR CYTOGENETIC STUDY OF THE NF2 GENE DELETION IN MENINGIOMA IN SUDANESE PATIENTS

Pubblicato online: 11 mar 2014

Pagine: 29 - 32

DOI: https://doi.org/10.2478/bjmg-2013-0028

Parole chiaveMeningioma, NF2 Gene, Tumor suppressor gene, Banding cytogenetics, Interphase fluorescence in situ hybridization (I-FISH), Sudan

This content is open access.

Parole chiave
Meningioma, NF2 Gene, Tumor suppressor gene, Banding cytogenetics, Interphase fluorescence in situ hybridization (I-FISH), Sudan