Comparison of three immunoassays for detection of anti-toxoplasma IgM antibodies in pregnant women
Pubblicato online: 14 dic 2023
Pagine: 13 - 22
DOI: https://doi.org/10.2478/bjir-2023-0014
Parole chiave
© 2023 Blerta Laze, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial 4.0 International License.
Aim of investigation
Comparison of an electrochemiluminescence technique (ECL, applied in Cobas 6000 instrument), an enzyme-linked immunosorbent assay (ELISA, applied in CHORUS instrument) and an enzyme-linked fluorescent assay (ELFA, applied in MINI-VIDAS instrument) for early diagnosis of
Methods
ECL, ELISA and ELFA techniques are used to detect anti-toxoplasma IgM antibodies in pregnant women, during the first trimester of pregnancy. 200 samples were analyzed with each technique and sensitivity and specificity are evaluated for each of them.
Results
ECL technique has resulted in higher sensitivity (100%) and specificity (99.3%), while ELISA technique has resulted in lower sensitivity (87.5%) and specificity (97.3%).
Conclusion
Analysis of the results confirmed the usefulness of ECL technique for an early diagnosis of