<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>This work is geared towards extraction, isolation and characterization of phytocompounds from the ethyl acetate extract of <italic>Acanthus montanus</italic> root. The ethyl acetate extract was obtained through sequential maceration using a nonpolar solvent first before introducing ethyl acetate. The extract was subjected to vacuum liquid chromatography (VLC) for fractionation and phytocompounds were purified through column chromatography. Characterization was done employing infrared spectroscopy (IR) and nuclear magnetic spectroscopy (NMR). The phytocompounds were isolated and characterized as 6,7-dihydroxy-4-methoxycoumarin and 2ʹ-acetoxy-4ʹ,5-dihydroxy-6ʹ-methoxy-3-(2ʹʹ-hydroxy-6ʹʹ-oxanyl)-8-(2ʹʹʹ-hydroxy-4ʹʹʹ-oxanyl)flavanone named Acanthusin G with percentage yield of 0.7% and 1.16% respectively. 6,7-dihydroxy-4-methoxycoumarin and Acanthusin G indicated good antimicrobial activities at 200 µg/ml with zone of inhibition range of 18 - 36 mm against fifteen pathogens using agar well diffusion method.</p>
</abstract>

This work is geared towards extraction, isolation and characterization of phytocompounds from the ethyl acetate extract of Acanthus montanus root. The ethyl acetate extract was obtained through sequential maceration using a nonpolar solvent first before introducing ethyl acetate. The extract was subjected to vacuum liquid chromatography (VLC) for fractionation and phytocompounds were purified through column chromatography. Characterization was done employing infrared spectroscopy (IR) and nuclear magnetic spectroscopy (NMR). The phytocompounds were isolated and characterized as 6,7-dihydroxy-4-methoxycoumarin and 2ʹ-acetoxy-4ʹ,5-dihydroxy-6ʹ-methoxy-3-(2ʹʹ-hydroxy-6ʹʹ-oxanyl)-8-(2ʹʹʹ-hydroxy-4ʹʹʹ-oxanyl)flavanone named Acanthusin G with percentage yield of 0.7% and 1.16% respectively. 6,7-dihydroxy-4-methoxycoumarin and Acanthusin G indicated good antimicrobial activities at 200 µg/ml with zone of inhibition range of 18 - 36 mm against fifteen pathogens using agar well diffusion method.

Bioassay-directed isolation of two novel antimicrobial coumarin and flavanone from <italic>Acanthus montanus</italic>

Ovidius University Annals of Chemistry

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Bioassay-directed isolation of two novel antimicrobial coumarin and flavanone from 

This work is geared towards extraction, isolation and characterization of phytocompounds from the ethyl acetate extract of Acanthus montanus root. The ethyl...

Bioassay-directed isolation of two novel antimicrobial coumarin and flavanone from Acanthus montanus

Pubblicato online: 17 lug 2024

Pagine: 67 - 72

Ricevuto: 28 mar 2024

Accettato: 29 giu 2024

DOI: https://doi.org/10.2478/auoc-2024-0009

Parole chiave
vacuum liquid chromatography, coumarin, flavanone, oxanol, Acanthusin G, pathogens

© 2024 Gloria Ihuoma Ndukwe et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Bioassay-directed isolation of two novel antimicrobial coumarin and flavanone from Acanthus montanus

Pubblicato online: 17 lug 2024

Pagine: 67 - 72

Ricevuto: 28 mar 2024

Accettato: 29 giu 2024

DOI: https://doi.org/10.2478/auoc-2024-0009

Parole chiavevacuum liquid chromatography, coumarin, flavanone, oxanol, Acanthusin G, pathogens

© 2024 Gloria Ihuoma Ndukwe et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Parole chiave
vacuum liquid chromatography, coumarin, flavanone, oxanol, Acanthusin G, pathogens