Chitosan, a biodegradable and nontoxic biopolymer, has applications in a wide range of fields. This study aimed to produce and characterize chitosan from three mushroom species obtained from Edo State, Nigeria. Standard protocols were used to extract and characterize chitosan. Chitosan yield from all three samples differed significantly (p<0.05) with the highest chitosan yield (19.00±0.03 %) from