<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Jojoba cultivation and production face the challenge of establishing ways to identify the sex at early stage of plant growth. The present study was carried out to identify sex of jojoba at the seedling stage under Sudan condition. Two DNA markers, ISSR (UBC807) and RAPD (OPG-5), were used for sex identification of jojoba genotypes: two known male and females genotypes and four unknown genotypes. ISSR marker, UBC807 was successfully amplified a unique male-specific band at 1200 bp, while RAPD marker, OPG-5 could not amplify a unique band within jojoba sex. The result clearly indicates that ISSR-UBC807 marker can be used for sex identification of jojoba at seedlings stage, a finding that could make the commercial cultivation and production of jojoba possible in Sudan.</p>
</abstract>

Jojoba cultivation and production face the challenge of establishing ways to identify the sex at early stage of plant growth. The present study was carried out to identify sex of jojoba at the seedling stage under Sudan condition. Two DNA markers, ISSR (UBC807) and RAPD (OPG-5), were used for sex identification of jojoba genotypes: two known male and females genotypes and four unknown genotypes. ISSR marker, UBC807 was successfully amplified a unique male-specific band at 1200 bp, while RAPD marker, OPG-5 could not amplify a unique band within jojoba sex. The result clearly indicates that ISSR-UBC807 marker can be used for sex identification of jojoba at seedlings stage, a finding that could make the commercial cultivation and production of jojoba possible in Sudan.

DNA Fingerprinting of Sex in Jojoba (<italic>Simmondsia chinensis</italic>) Grown under the Semi-arid Conditions of Sudan

Department of Botany and Agricultural biotechnology, Faculty of Agriculture

Department of Agronomy, Faculty of Agriculture

Advanced Research in Life Sciences

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

DNA Fingerprinting of Sex in Jojoba () Grown under the Semi-arid Conditions of Sudan

Jojoba cultivation and production face the challenge of establishing ways to identify the sex at early stage of plant growth. The present study was carried out...

DNA Fingerprinting of Sex in Jojoba (Simmondsia chinensis) Grown under the Semi-arid Conditions of Sudan

Pubblicato online: 10 giu 2019

Pagine: 23 - 27

Accettato: 01 mag 2019

DOI: https://doi.org/10.2478/arls-2019-0006

Parole chiave
DNA markers, Goat nut, Male/Female identification, ISSR, RAPD

© 2019 Ismail A. Mohammed et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

DNA Fingerprinting of Sex in Jojoba (Simmondsia chinensis) Grown under the Semi-arid Conditions of Sudan

Pubblicato online: 10 giu 2019

Pagine: 23 - 27

Accettato: 01 mag 2019

DOI: https://doi.org/10.2478/arls-2019-0006

Parole chiaveDNA markers, Goat nut, Male/Female identification, ISSR, RAPD

© 2019 Ismail A. Mohammed et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Parole chiave
DNA markers, Goat nut, Male/Female identification, ISSR, RAPD