Efficient, cost-effective DNA extraction methods are crucial for molecular research on sturgeon embryos given that substantial sample sizes must frequently be analyzed in short periods of time. The high lipid and carbohydrate contents of sturgeon embryo yolk sacs mean that obtaining genetic material of sufficient quality is challenging. The predominant methods used include tissue/cell lysis, organic extraction, purification on spin columns, and ethanol precipitation. However, these methods are expensive and time-consuming, which significantly limits the throughput of PCR-based molecular analyses. In the present study, we evaluated the usefulness of an in-house Chelex-100 DNA extraction method on sterlet (