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Australasian Orthodontic Journal
Volume 39 (2023): Numero 2 (July 2023)
Accesso libero
Unbalanced cartilage calcification during development contributes to the formation of irregular articular surfaces as revealed by micro-CT images
Yuejiao Zhang
Yuejiao Zhang
,
Jing Zhang
Jing Zhang
,
Lingfeng Xu
Lingfeng Xu
e
Meiqing Wang
Meiqing Wang
| 21 ago 2023
Australasian Orthodontic Journal
Volume 39 (2023): Numero 2 (July 2023)
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Pubblicato online:
21 ago 2023
Pagine:
40 - 48
Ricevuto:
01 feb 2023
Accettato:
01 lug 2023
DOI:
https://doi.org/10.2478/aoj-2023-0025
© 2023 Yuejiao Zhang et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.
Figure 1.
Representative frontal and lateral views, and a sketch map of the incisors’ occlusal relationship in experimental group rats. BAE, bilateral anterior elevation; BAC, bilateral anterior crossbite; CON, blank control; GIDO, gradually induced disordered occlusion; MDO, mandibular deviated occlusion.
Figure 2.
The timeline of the entire experiment.
Figure 3.
Morphology of the lacunae revealed by gross observation, micro-CT images, and toluidine blue (TB) and von Kossa (VK) staining. For Gross morphology, micro-CT images, TB and VK staining images, bars = 500 μm; for HE staining images, bars = 100 μm.
Figure 4.
Representative histochemistry, immune-histochemistry (IHC) staining of the condylar lacuna induced by occlusal disturbance. Lacunae were detected by SO staining and IHC staining for Col-II, Col-X, OSX. For SO and IHC staining, bars = 100 μm.
Figure 5.
Representative sections with condylar lacunae induced by occlusal disturbance, stained with Tartrate-resistant acid phosphatase (TRAP) and Safranin O (SO), immune-histochemistry (IHC) for FN and immunofluorescence (IF) for cleaved caspase-3 (CCP3) and CD90. The black frames in the SO staining images showed the selected lacunae regions for IF staining. For SO, TRAP and IHC staining, bars = 100 μm. For IF staining, bars = 100 μm.
Figure 6.
The diagrammatic sketch for normal growth of the condyle (A) and lacunae formation (B).