<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>The aetiology and pathogenesis of many canine tumours are likely to be similar to cancers found in humans. This study aimed to evaluate a plausible link between changes in the <italic>tRNA-Leu (UUR)</italic> gene and the carcinogenesis process in dogs with mammary gland tumours. The whole mitochondrial DNA (mtDNA) isolated from blood and tumour tissues of 13 dogs with malignant mammary gland tumours was sequenced. The present work is the first report showing that some polymorphisms might occur at the corresponding positions in the human and canine mtDNA genome, which in turn may provoke similar deleterious effects. The homology between the human <italic>MT-TL1</italic> and canine <italic>tRNA-Leu (UUR)</italic> genes was 84%. After resequencing of the whole mitochondrial DNA genome with the use of the NGS technology, two polymorphisms in two haplotypes were identified: m.2683G>A (observed in 18 out of 27 samples) and m.2678_2679insG (27 out of 27 samples). The m.2683G>A polymorphism corresponded to a deleterious change at m.3243A>G, which is linked with MELAS (Mitochondrial Encephalomyopathy, Lactic Acidosis, Stroke-like episodes) syndrome and with different types of cancers in humans as well. The comparative analysis of <italic>MT-TL1</italic> and <italic>tRNA-Leu (UUR)</italic> led us to hypothesise that the m.2678_2679insG and m.2683G>A polymorphisms might influence the dog’s condition and might be linked with tumourigenesis, as observed in humans.</p>
</abstract>

The aetiology and pathogenesis of many canine tumours are likely to be similar to cancers found in humans. This study aimed to evaluate a plausible link between changes in the tRNA-Leu (UUR) gene and the carcinogenesis process in dogs with mammary gland tumours. The whole mitochondrial DNA (mtDNA) isolated from blood and tumour tissues of 13 dogs with malignant mammary gland tumours was sequenced. The present work is the first report showing that some polymorphisms might occur at the corresponding positions in the human and canine mtDNA genome, which in turn may provoke similar deleterious effects. The homology between the human MT-TL1 and canine tRNA-Leu (UUR) genes was 84%. After resequencing of the whole mitochondrial DNA genome with the use of the NGS technology, two polymorphisms in two haplotypes were identified: m.2683G>A (observed in 18 out of 27 samples) and m.2678_2679insG (27 out of 27 samples). The m.2683G>A polymorphism corresponded to a deleterious change at m.3243A>G, which is linked with MELAS (Mitochondrial Encephalomyopathy, Lactic Acidosis, Stroke-like episodes) syndrome and with different types of cancers in humans as well. The comparative analysis of MT-TL1 and tRNA-Leu (UUR) led us to hypothesise that the m.2678_2679insG and m.2683G>A polymorphisms might influence the dog’s condition and might be linked with tumourigenesis, as observed in humans.

Evaluation of the <italic>tRNA-Leu (UUR)</italic> gene haplotype profile observed in canine mammary gland tumours based on comparative analysis with the <italic>MT-TL1</italic> human gene

Department of Genomics and Biodiversity, Institute of Genetics and Animal Biotechnology

Annals of Animal Science

This work is licensed under the Creative Commons Attribution 4.0 International License.

Evaluation of the  gene haplotype profile observed in canine mammary gland tumours based on comparative analysis with the  human gene

The aetiology and pathogenesis of many canine tumours are likely to be similar to cancers found in humans. This study aimed to evaluate a plausible link...

Evaluation of the tRNA-Leu (UUR) gene haplotype profile observed in canine mammary gland tumours based on comparative analysis with the MT-TL1 human gene

Pubblicato online: 19 lug 2022

Pagine: 915 - 922

Ricevuto: 11 giu 2021

Accettato: 13 dic 2021

DOI: https://doi.org/10.2478/aoas-2022-0009

Parole chiave
polymorphisms, mtDNA, mammary gland tumours, MELAS

© 2022 Krzysztof Kowal et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Evaluation of the tRNA-Leu (UUR) gene haplotype profile observed in canine mammary gland tumours based on comparative analysis with the MT-TL1 human gene

Pubblicato online: 19 lug 2022

Pagine: 915 - 922

Ricevuto: 11 giu 2021

Accettato: 13 dic 2021

DOI: https://doi.org/10.2478/aoas-2022-0009

Parole chiavepolymorphisms, mtDNA, mammary gland tumours, MELAS

© 2022 Krzysztof Kowal et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Parole chiave
polymorphisms, mtDNA, mammary gland tumours, MELAS